hybridoma


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hybridoma

 [hi″brĭ-do´mah]
a somatic cell hybrid formed by fusion of normal lymphocytes and tumor (lymphoma) cells. B cell hybridomas are the most useful source of monoclonal antibodies; the hybridoma cells are able to produce antibody like the normal parent and to proliferate indefinitely in culture like the parent tumor cells.

hy·brid·o·ma

(hī'brid-ō'mă),
A tumor of hybrid cells used in the in vitro production of specific monoclonal antibodies; produced by fusion of an established tissue culture line of lymphocyte tumor cells (for example, mouse plasmacytoma cells) and specific antibody-producing cells (for example, splenocytes from specifically immunized mice). Fusions are accomplished by use of polyethylene glycol or other methods.
[G. hybris, violation, wantonness, + -ōma, tumor]

hybridoma

/hy·brid·o·ma/ (hi″brid-o´mah) a somatic cell hybrid formed by fusion of normal lymphocytes and tumor cells.

hybridoma

(hī′brĭ-dō′mə)
n.
A cell that is produced in the laboratory by fusing an antibody-producing lymphocyte (which does not readily divide) with a nonantibody-producing cancer cell (which divides rapidly). The hybridoma proliferates and produces a continuous supply of a specific monoclonal antibody.

hybridoma

[hī′bridō′mə]
a hybrid cell formed by the fusion of a myeloma cell and an antibody-producing cell. Hybridomas are used to produce monoclonal antibodies.

hy·brid·o·ma

(hī'brid-ō'mă)
A tumor of hybrid cells used in the production in vitro of specific monoclonal antibodies; produced by fusion of an established tissue culture line of lymphocyte tumor cells and specific antibody-producing cells.
[G. hybris, violation, wantonness, + -ōma, tumor]

hybridoma

A cell line (CLONE) formed by the fusion of two or more different types of cells, such as a B LYMPHOCYTE and a lymphoid tumour cell (myeloma). Malignant tumours can be immortal so a hybridoma, suitably nourished, can continue permanently exercising the function of the B lymphocyte to produce specific antibodies (MONOCLONAL ANTIBODIES).

hybridoma

a cell made by fusing an antibody-producing B-CELL with a CANCER cell. The cancer cell contributes the ability to divide indefinitely, while the antibody cell contributes the ability to synthesize large amounts of a single antibody A hybridoma CLONE forms as a result of division of this hybrid, and can be maintained indefinitely in culture, producing large amounts of pure antibody The antibodies produced by a specific hybridoma clone are identical, they are MONOCLONAL ANTIBODIES.

hybridoma

a cell culture consisting of a clone of a hybrid cell formed by fusing cells of different kinds. Hybridomas formed from mouse B lymphocytes following inoculation of a particular antigen and myeloma cells are immortal and produce monoclonal antibodies to the inoculated antigen. Such antibodies have produced a revolution in the level at which antibody-antigen reactions can be analyzed leading to precise definition of important epitopes, to methods for purifying antigens, improved diagnostic methods and, it is anticipated, to new methods of therapy.

interspecific hybridoma
one formed by fusion of cells from two different species, such as mouse and rat or hamster.
References in periodicals archive ?
We used VH and VL genes isolated from the hybridoma cell line to construct a scFv antibody for in vitro directed evolution.
Selective HAT medium (Sigma-Aldrich, Germany) was then used for selection of hybridoma cells.
Because none of the 14 mAbs reacted with the denatured fragments NNDC1 or NT3, we did not obtain hybridoma antibodies raised against the linear epitope located in the region containing amino acids 121-213.
Kresina and his co-workers are using a common animal model of arthritis to study the effect of so-called hybridomas on the disease.
Validation of the hybridoma cell line in terms of antibody production, antibody isotyping and antigen-binding specificity;
Under the terms of today's agreement, Ventana gains exclusive access from Clavis Pharma to the hENT1 antibody and hybridoma line around which Ventana will seek to develop an immuno-histochemistry (IHC) assay for use in Clovis Oncology's clinical trials to identify patients with low level of hENT1 expression in the tumour.
L] genes from a murme hybridoma to the constant region heavy and light chain genes derived from a human myeloma cell line.
The system satisfies multiple applications: serial dilutions, mixing, storage, and harvesting of Hybridoma cells, growing of cells for cell culture assays, freezer storage and most EIA/ELISA and RIA procedures.
Sacks, who discussed his research at this month's Recombinant DNA/ Hybridoma Congresses in San Francisco, is one of a growing number of scientists who are using specific components of a parasite to induce immune responses.
The platform will support biopharma R&D operations from early B-cell and hybridoma screening, to antibody engineering, expression, purification, and analytics.