hybridoma


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hybridoma

 [hi″brĭ-do´mah]
a somatic cell hybrid formed by fusion of normal lymphocytes and tumor (lymphoma) cells. B cell hybridomas are the most useful source of monoclonal antibodies; the hybridoma cells are able to produce antibody like the normal parent and to proliferate indefinitely in culture like the parent tumor cells.
Miller-Keane Encyclopedia and Dictionary of Medicine, Nursing, and Allied Health, Seventh Edition. © 2003 by Saunders, an imprint of Elsevier, Inc. All rights reserved.

hy·brid·o·ma

(hī'brid-ō'mă),
A tumor of hybrid cells used in the in vitro production of specific monoclonal antibodies; produced by fusion of an established tissue culture line of lymphocyte tumor cells (for example, mouse plasmacytoma cells) and specific antibody-producing cells (for example, splenocytes from specifically immunized mice). Fusions are accomplished by use of polyethylene glycol or other methods.
[G. hybris, violation, wantonness, + -ōma, tumor]
Farlex Partner Medical Dictionary © Farlex 2012

hybridoma

(hī′brĭ-dō′mə)
n.
A cell that is produced in the laboratory by fusing an antibody-producing lymphocyte (which does not readily divide) with a nonantibody-producing cancer cell (which divides rapidly). The hybridoma proliferates and produces a continuous supply of a specific monoclonal antibody.
The American Heritage® Medical Dictionary Copyright © 2007, 2004 by Houghton Mifflin Company. Published by Houghton Mifflin Company. All rights reserved.

hy·brid·o·ma

(hī'brid-ō'mă)
A tumor of hybrid cells used in the production in vitro of specific monoclonal antibodies; produced by fusion of an established tissue culture line of lymphocyte tumor cells and specific antibody-producing cells.
[G. hybris, violation, wantonness, + -ōma, tumor]
Medical Dictionary for the Health Professions and Nursing © Farlex 2012

hybridoma

A cell line (CLONE) formed by the fusion of two or more different types of cells, such as a B LYMPHOCYTE and a lymphoid tumour cell (myeloma). Malignant tumours can be immortal so a hybridoma, suitably nourished, can continue permanently exercising the function of the B lymphocyte to produce specific antibodies (MONOCLONAL ANTIBODIES).
Collins Dictionary of Medicine © Robert M. Youngson 2004, 2005

hybridoma

a cell made by fusing an antibody-producing B-CELL with a CANCER cell. The cancer cell contributes the ability to divide indefinitely, while the antibody cell contributes the ability to synthesize large amounts of a single antibody A hybridoma CLONE forms as a result of division of this hybrid, and can be maintained indefinitely in culture, producing large amounts of pure antibody The antibodies produced by a specific hybridoma clone are identical, they are MONOCLONAL ANTIBODIES.
Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005
References in periodicals archive ?
Yagci, "Immunization with UV-induced apoptotic cells generates monoclonal antibodies against proteins differentially expressed in hepatocellular carcinoma cell lines," Hybridoma, vol.
Selection of positive hybridomas, i.e., those that produced adenovirus antigen-specific antibodies was performed by indirect ELISA.
Hybridoma cell culture in vitro has become an essential molecular tool in basic cell and biotechnological research.
After the cells were thawed, the human/mouse hybridomas were seeded into a 75 [cm.sup.2] bottle and allowed to grow to 80% confluency.
We characterized an anti-pGHR antibody (denoted by AN98) using the hybridoma technique.
(2006) Production of a mouse hybridoma secreting monoclonal antibody highly specific to hemoglobin Bart's (gamma4).
We used special sterile plastic dishes for cell culture in order to culture hybridomas: Petri dishes of 96-well flat bottom plates, pipettes, Petri dishes, mattresses with the growth area of 25 [cm.sup.2] (Nunc, Denmark).
Hybridoma.--Hybridomas producing antibodies to SOX10 were generated by standard techniques from splenocytes of SOX10-immunized BALB/c mice.
After generation of hybridomas, we identified clones producing antibody against WU-VP1 by ELISA and immunoblot.
It is now able to offer new services, including antibody humanisation to supplement its antibody engineering, antibody expression and hybridoma sequencing services.
More than 120 scientific papers, most of which revolve around cancer and blood disorders, stem cells, genetic diseases, parasitic diseases, immunology and tissue culture hybridoma, genome and biotechnology will be presented.
MAbs recognizing FFL(Firefly luciferase) specificly were developed by obtaining hybridoma cell lines from the immunized animals injected with the firefly luciferase protein .