hybridization


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Related to hybridization: DNA hybridization

hybridization

 [hi″brid-ĭ-za´shun]
1. the production of hybrids.
fluorescent in situ hybridization (FISH) a genetic mapping technique using fluorescent tags for analysis of chromosomal aberrations and genetic abnormalities. Called also chromosome painting.
molecular hybridization in molecular biology, formation of a partially or wholly complementary nucleic acid duplex by association of single strands, usually between DNA and RNA strands or previously unassociated DNA strands, but also between RNA strands; used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands.

hy·brid·i·za·tion

(hī'brid-i-zā'shŭn),
1. The process of breeding a hybrid.
2. Crossing over between related but nonallelic genes.
3. The specific reassociation of complementary strands of polynucleic acids, for example, the formation of a DNA-RNA hybrid.
4. The process or act of forming a macromolecular hybrid in which the subunits are obtained from different sources.
Synonym(s): crossbreeding

hybridization

/hy·brid·iza·tion/ (hi″brid-ĭ-za´shun)
1. crossbreeding; the act or process of producing hybrids.
3. formation of a heterokaryon by fusion of two somatic cells, usually of different species.
4. in chemistry, a procedure whereby orbitals of intermediate energy and desired directional character are constructed.

in situ hybridization  molecular hybridization used to analyze prepared cells or histologic sections in situ in order to analyze the intracellular or intrachromosomal distribution, transcription, or other characteristics of specific nucleic acids.
molecular hybridization  formation of a partially or wholly complementary nucleic acid duplex by association of single strands, in order to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands.

hybridization

[hī′bridīzā′shən]
1 the process of producing hybrids by crossbreeding.
2 the process of combining single-stranded nucleic acids from different sources to form stable, double-stranded molecules. The technique involves fragmentation and separation of the source nucleic acids by heating, followed by recombination through cooling. The resulting hybrids can be DNA-DNA, DNA-RNA, or RNA-RNA duplexes.

hybridization

Molecular biology The formation of a complex of complementary nucleotides; hybridization allows determination of the relatedness or sequence 'homology' between 2 strands of nucleic acids, and precise ID of short–up to 20 kb segments of DNA–Southern blot or RNA–Northern blot

hy·brid·i·za·tion

(hī'brid-ī-zā'shŭn)
1. The process of breeding a hybrid.
2. Crossing over between related but nonallelic genes.
3. The specific association of complementary strands of polynucleic acids, e.g., the formation of a DNA-RNA hybrid.
Synonym(s): hybridisation.

hybridization

See MOLECULAR HYBRIDIZATION.

hy·brid·i·za·tion

(hī'brid-ī-zā'shŭn)
1. The process of breeding a hybrid.
2. Crossing over between related but nonallelic genes.
Synonym(s): hybridisation.

hybridization

the production of hybrids.

in situ hybridization of nucleic acid
a fragment of radioisotope or otherwise labeled DNA can be used as a probe to detect related nucleic acid sequences in cells or tissues. The method requires that both the probe and the cellular nucleic acid sequences be melted to single-stranded forms and then allowed to form either DNA:DNA or DNA:RNA double-stranded forms.
interspecies hybridization
see interspecific hybridoma.
nucleic acid hybridization
double-stranded DNA when heated in aqueous solution separates into two single strands in a process called denaturation or melting. The single strands readily reform a double helix when the solution is allowed to cool in a process called DNA renaturation. Hybridization will occur between any two single-stranded nucleic acid molecules (DNA:DNA, RNA:RNA, RNA:DNA) provided they have complementary nucleotide sequences. The degree of homology between any two nucleic acid molecules can be measured by the percentage of base pairing.
References in periodicals archive ?
We can now better understand the opinions of Maharal and Ibn Ezra, which related the general prohibition against hybridization to the concept of sexual morality.
All cases genotyped by fluorescent hybridization probes agreed with PCR restriction fragment length analysis.
Keehner also wants to know if long-term changes in seasonal weather patterns are increasing the rate of hybridization.
Hybridization is superior to culture screening methods and largely complimentary to PCR, but has a potentially broader epidemiologic application since it is unaffected by minor sequence variations that can completely inhibit PCR.
Parental origin of the extra chromosome in the cat eye syndrome: Evidence from heteromorphism and in situ hybridization analysis.
Hybridization is a method of placing a known gene on a coverslip slide and then probing it with unknown genes.
0-litre conventional diesel engine and offers possible total weight savings of up to 30%, thereby compensating for the additional weight normally expected in mild hybridization.
Many methods of detection of hybridization have been proposed.
The development of commercial-scale hybridization techniques for our major agronomic crops has been hailed as one of the greatest breakthroughs in agricultural history.
The two patents cover different aspects of the company's DNA analysis technologies, thermodynamic approach to Ligand Controlled Hybridization and sequence selection for DNA binding ligands.