Expression of housekeeping genes
in the gonad of carps
as internal standards: use and limits.
To identify and validate housekeeping genes
in the brains of the desert locust, schistocerca gregaria Forsskal (Acrididae) under various developmental conditions, Van Hiel et al.
Effect of experimental treatment on housekeeping gene
expression: validation by real-time, quantitative RT-PCR.
pneumoniae strains to compare them and confirm clonal and pathogenicity relationships through housekeeping gene
This analysis uses the difference between the housekeeping gene
and the control sample as [sup.
The idea of a housekeeping gene
is that it should be uniformly expressed throughout all the experimental or environmental conditions of a given system (14).
The researchers found that these induced genes, in contrast to the 'clueless' housekeeping genes
, act in an expected (well-regulated) way.
Seven genes having high similarity to housekeeping genes
from different Clostridium species were identified.
Transposable elements, the promoter regions of housekeeping genes
, and cis-acting regulatory elements of imprinted genes are three key epigenetic susceptibility targets containing CpG sites that are normally methylated, unmethylated, and differentially methylated, respectively.
However, this may not be the case in all microarray experiments, and thus other genes such as housekeeping genes
(with constant expression) may facilitate controlling other sources of variability (Yang et al.
One user-assigned target, three housekeeping genes
, as well as one procedural control are incorporated in each well.