housekeeping genes


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house·keep·ing genes

genes that are generally always expressed and thought to be involved in routine cellular metabolism.

housekeeping genes

Constitutive genes, genes that provide the basic routine functions required by all cells for their sustenance.

housekeeping genes

GENES governing the fundamental metabolic processes of CELLS and that are EXPRESSED in most cell types.
References in periodicals archive ?
Identification of genes from flat oyster Ostrea edulis as suitable housekeeping genes for quantitative real time PCR.
In this study, the 152 strains were further analyzed by the reference methods, including molecular biological analysis (16S rRNA gene sequence analysis and housekeeping gene sequence analysis), and physiological and biochemical analysis if necessary.
(2004) Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations Biotechnology Letter 26, 509-515.
To ensure the integrity of these results, the additional housekeeping gene 18S rRNA was used as an internal standard to demonstrate that ACTB and PBGD mRNAs were not differentially regulated in the groups of GCs.
[DELTA]Ct values were normalized using the mean values of three housekeeping genes: [beta]-Actin, [beta]-2-microglobulin, and GAPDH.
The melting curves of the 2 housekeeping genes ACTB and HTN3 showed a single peak with similar melting temperatures (85 [degrees]C), indicating no DNA contamination and no primer-dimer artifacts.
Based on earlier gene expression studies in domestic fowl, we have tested the stability of expression of seven housekeeping genes, including GAPDH, ACTB, HPRT1, TUB, succinate dehydrogenase flavoprotein A (SDHA), 18S rRNA and 28S rRNA in this study.
In Chapter 3, the authors cover topics such as the quality and quantity of the labeled target, the use of housekeeping genes and other controls, imaging of the arrays, and initial statistical and data analysis steps.
To characterize the Leptospira species detected in bats from Mengyin County, we attempted multilocus sequence typing (MLST) on 7 housekeeping genes (glmU, pntA, sucA, tpiA, pfkB, mreA, and caiB), as previously described (9).
Specific primers for determining the expression of housekeeping genes and the genes of interest (Table 1) were designed using Primer 3 software.