To show the variation of each housekeeping gene
under the toxicity stress in whole bodies or midguts, the most stable gene, EF1[alpha], was used as the reference to normalize the expressions and to calculate the fold of change of expression of each gene.
A set of 8 different RNA tumor markers (Table 3) containing putative as well as established bladder cancer markers and the housekeeping gene
GAPDH were quantified as specified in Fig.
Relative expression results may be influenced by interindividual differences in housekeeping gene
In the UROtsa cell line, hsp 27 mRNA was highly expressed, with mRNA being detected 10-15 cycles earlier in the PCR than were the g3pdh housekeeping gene
(Rossi et al.
Hence three different software tools were used to validate the stability of the selected housekeeping genes
in different developmental stages (1 d, 2, 4, 6 and 8 months old) of Zi geese tissues (heart, liver, kidney, muscle, ovary) using real-time qRT-PCR.
The mGAPDH mean Ct values obtained with the human wild-type [beta]-globin was normalized alternatively with either housekeeping gene
Total RNA was isolated from confluent UROtsa cells grown on both serum-containing and serum-free growth medium and used to determine the basal expression of the respective heat shock protein genes relative to the glyceraldehyde 3-phosphate dehydrogenase (g3pdh) housekeeping gene
using RTPCR technology as described previously (23-25).
The sequence and amplicon sizes of the primers are shown in Table 1 with 18S rRNA as the housekeeping gene
Pre designed primers and probes for c-Myc gene were selected and purchased from Applied Bio System and for data normalization, GAPDH housekeeping gene
primer was custom designed using primer express software (ABI) (Table II).
Apoptotic effects by different glucose level were determined by real-time, reverse transcription-PCR and GAPDH was use as the endogenous housekeeping gene
Hypoxanthine guanine phosphoribosyltransferase (HPRT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as housekeeping gene
and data were normalized relative to the expression level of the HPRT gene.
The disease occurs when a faulty housekeeping gene
fails to remove cell waste, like lipids and cholesterol.