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After washing to remove all traces of Percoll, the myocytes were cultured in DMEM containing 5% fetal calf serum, penicillin and streptomycin (P/S, 1%), and 10% horse serum for 48 h (15).
According to assay results, 51 (55%) horse serum samples were Ehrlichia spp.
They were then re-suspended in Dulbecco' modified Eagle' medium (DMEM), with 10% (v/v) heat-inactivated horse serum (HS), 10% fetal calf serum (FCS) and gentamicin (40 [micro]g/mL).
The growth factors are either supplied from FBS, horse serum, goat serum, human adult blood serum or purified growth factors from fetal bovine, goat, horse and human sera.
Additional information on horse serum antivenin comes from studies of Australian redback spider bites.
In addition to cross-reactivity with monkey serum, one of the MAbs (3F2D8) was cross-reacted with dog serum and another one (1F4C4) cross-reacted with horse serum.
UK, (TCSBiosciences online) has pioneered processes that guarantee consistent and high quality donor horse serum.
This reaction is due to the horse serum used and the quality control in the manufacture of the product.
pylori strains were cultured in brucella broth that was supplemented with 5% horse serum at 37 C under a 10% CO(2)atmosphere.
Opacity factor was determined by taking 10 [micro]l of overnight culture supernatant, added to the wells of microplate having 100 [micro]l of inactivated horse serum (15).
As an alternative to HRIG, equine immune globulin derived from horse serum is used in many parts of the world, but it is also scarce, expensive and can carry significant side effects.