Pangene invented, patented and commercializes enhanced Homologous Recombination
technologies that enhance the natural process of homologous recombination
used by cells to repair and recombine DNA.
and non-homologous end-joining pathways of DNA double-strand break repair have overlapping role in the maintenance of chromosomal integrity in vertebrate cells.
One possible explanation is that the cellular capacity for DNA repair by homologous recombination
in these two tumour cell clones is finite resulting in their similar SCE levels despite the significant difference in the endogenous DSBs.
Doetschman et al., "Germ-line transmission of a planned alteration made in a hypoxanthine phosphoribosyltransferase gene by homologous recombination
in embryonic stem cells," Proceedings of the National Academy of Sciences of the United States of America, vol.
Rubraca is an oral, small molecule inhibitor of PARP enzymes, including PARP1, PARP-2, and PARP-3, being developed for the treatment of solid tumors associated with homologous recombination
deficiency (HRD), defined as the presence of a deleterious BRCA1 or BRCA2 mutation, a deleterious mutation in another gene involved in DNA damage repair, and/or a high percentage of tumor genome with LOH, a phenotypic consequence of HRD.
Gene targeting by homologous recombination
in mouse zygotes mediated by zinc-finger nucleases.
Cleaved DNA ends were then re-united to each other either through non homologous recombination
(NHR) or homologous recombination
Specifically, it is involved in homologous recombination
(a form of double-stranded DNA repair mechanism).
A prominent mechanism for genomic diversity and rearrangement of genetic material in prokaryotes is general homologous recombination
. Homologous recombination
generally requires long stretch of homologous DNA that is >50 base pair in length and relies on number of DNA repair and cell maintenance machinery proteins.
, for instance, is an integral feature of important repair pathways including the synthesis-dependent strand-annealing pathway of double strand break repair .
Gene knockout via homologous recombination
is a powerful tool for the generation of specific mutants and subsequent functional analysis of the gene.
One of the last steps in chromosome segregation before cell division involves the resolution of dimeric chromosomes that are frequently produced by homologous recombination
between sister chromatids following DNA damage .