The functional predictions are drawn from domain and Gene Ontology (GO) assignments for homologous proteins
derived from over 15 years of manual curation from peer-reviewed literature on mammalian, yeast, worm, and plant species.
This technology ensures complete absence of homologous proteins
and other contaminants in the final preparation.
We used a BLASTP search to determine the similarity of predicted R1101 proteins to homologous proteins
5) The pollens and foods involved usually are not botanically related but contain homologous proteins
, as there are shared binding sites (epitopes) in the structures of pollen and food allergens.
This invention also relates to methods of using the structure coordinates to solve the structure of homologous proteins
or protein complexes.
In a phylogenetic analysis of the amino-acid sequences, the proteins coded for by the S1 segment, P10, P17, and sigma C, group with their homologous proteins
in other avian reoviruses, whereas the major capsid protein, sigma B.
Antibodies formed to species-specific protein components typically bind only to the proteins particular to that species, but homologous proteins
(with similar structures) may also exist in related or unrelated species.
Algorithms, enabling the appropriate weighting of the structural information extracted from the various remote homologous proteins
, are a key component of the new method, resulting in the prediction of previously unknown conformations for the target protein.
Examples of relevance to the NIDDK include new highly differentiated cell lines (such as tubule cells), mutant organisms, electrophysiologic or imaging methods to study transporter physiology and regulation in vivo, structure-function studies of purified homologous proteins
or proteins in model membrane systems, identification of human homologues to proteins studied in model organisms, and the search for novel genes and proteins involved in membrane transport of ions and nutrients.
It has been used extensively to understand enzymatic function evolution, in the study of sequence and structure variability and its dependence in homologous proteins
The majority of positive antibody reactions were directed toward only 1 of the 2 homologous proteins
Vaccinex utilizes its ActivMAb technology to directly generate fully functional antibodies against difficult targets such as homologous proteins
and multi-pass membrane receptors.