Catalase activity (figure 4) did not exhibit any statistically significant difference between the liver and lung homogenates
The CK activity only in the homogenate
was determined spectrophotometrically with commercial CK kit (Nanjing Jiancheng Bioengineering Institute, China).
Pools positive in the screening assay were confirmed by reextracting RNA from the original tick homogenate
and performing the quantitative rRT-PCR with 2 primer/probe sets, primer/probe sets NP1 and PB1.
The concentration of the GSH and some oxidative stress-related factors were also detected by ELISA in the hepatocyte homogenates
Duplicates of 10 [micro]l homogenates
were taken after centrifugation and plated in 96-well plates; 300 [micro]l of the Bradford reagent 1:5 diluted was added to each well.
75 [micro]g adipocyte homogenate
was applied directly, without boiling, to a nondenaturing 10% polyacrylamide gel.
Seawater was included as an additional variable in the plots for homogenates
Kidney, testis, spleen and jejunum were weighed and homogenized immediately to give 50% (w/v) homogenate
in ice- cold medium containing 50 mM TrisHCl and 300 mM sucrose, pH 7.
Metabolism experiments with mice intestine homogenate
Our observation of elevated activities of both SOD and catalase in whole body homogenates
of flies after 12 days of intoxication with PQ, suggests that flies were exposed to elevated oxidative stress in vivo, and that the damage persists for several days after intoxication.
Neither G7 nor G3 CAc expression was affected by injection of homogenates
Determined at 41[degrees]C by incubating 40 [micro]l of duodenal homogenate
with 200 gl of 1 mM inositolhexaphosphoric sodium salt and 200 [micro]l of 25 mM Mg[Cl.