Sulfur Indole Motility (SIM) medium indicated that samples Harlan surface (1), (2), and whole body homogenates
of bed bugs from Maryland and Colorado did not reduce sulfur, produce indole, and were not motile.
Determination of protein concentration was performed in liver homogenate
by the method of Lowry et al,  with bovine serum albumin as standard and expressed as mg/ml.
The compatibility of berry pulps and homogenates
with microwave-assisted sterilization was demonstrated within the temperature ranges of 20 C to 90 C using the dielectric property measurements that had been obtained, along with the 5-kW microwave heating system.
We measured levels of TNF[alpha], inrer-leukin-6 (IL-6), macrophage inflammatory protein-1[alpha] (MIP-1[alpha]), IL-1[beta], fractalkine, and RAGE (receptor for advanced glycation end products) in cell culture supernatant and brain homogenate
using commercially available ELISA kits (R&D Systems, Minneapolis, MN).
Zymogram of adult and prepubertal quail testes homogenate
revealed only single band at identical position when subjected to LDH enzyme specific stain (Figure 2).
were serially diluted and 2-[micro]L aliquots of each dilution were spot-plated onto NA to estimate the number of in vivo produced endospores.
Measurement of proportions of active and inactive forms of acetyl-CoA crboxylase The proportion of polymeric (active) and of the protomeric (inactive) forms of acetyl-CoA carboxylase in the nuclear-free and cytosolic fractions of lactating rat mammary gland were measured by preparing homogenates
of tissue, as described above, in the absence (that is with both the active polymeric and the inactive protomeric forms present) or in the presence (that is with only the active polymeric form present) of 400 mg avidin .
Approximately 7 ml of saline or homogenate
was added to the syringe and anaesthetic gas mixture (containing 5% halothane, 2.
The node homogenates
cannot be stored indefinitely and this approach allows permanent archival material to be stored in paraffin wax.
Cytotoxicity test: Cytotoxic factor (CF) is present in the homogenates
prepared from the DV-infected mouse spleen and has the capacity to kill normal mouse spleen cells in one hour time.
were prepared at 0-4 [degrees]C as previously described (Rothermel et al.
In our assay, the addition of an optimal concentration of BSA to the reaction mixture, combined with the use of isolated mitochondrial preparations instead of crude sample homogenates
, resulted in a complex I activity that was linear in time and had high rotenone sensitivity.