Catalase activity (figure 4) did not exhibit any statistically significant difference between the liver and lung
homogenates.
The CK activity only in the
homogenate was determined spectrophotometrically with commercial CK kit (Nanjing Jiancheng Bioengineering Institute, China).
Pools positive in the screening assay were confirmed by reextracting RNA from the original tick
homogenate and performing the quantitative rRT-PCR with 2 primer/probe sets, primer/probe sets NP1 and PB1.
The concentration of the GSH and some oxidative stress-related factors were also detected by ELISA in the hepatocyte
homogenates.
Duplicates of 10 [micro]l
homogenates were taken after centrifugation and plated in 96-well plates; 300 [micro]l of the Bradford reagent 1:5 diluted was added to each well.
75 [micro]g adipocyte
homogenate was applied directly, without boiling, to a nondenaturing 10% polyacrylamide gel.
Seawater was included as an additional variable in the plots for
homogenates.
Kidney, testis, spleen and jejunum were weighed and homogenized immediately to give 50% (w/v)
homogenate in ice- cold medium containing 50 mM TrisHCl and 300 mM sucrose, pH 7.
Metabolism experiments with mice intestine
homogenateOur observation of elevated activities of both SOD and catalase in whole body
homogenates of flies after 12 days of intoxication with PQ, suggests that flies were exposed to elevated oxidative stress in vivo, and that the damage persists for several days after intoxication.
Neither G7 nor G3 CAc expression was affected by injection of
homogenates (Fig.
Determined at 41[degrees]C by incubating 40 [micro]l of duodenal
homogenate with 200 gl of 1 mM inositolhexaphosphoric sodium salt and 200 [micro]l of 25 mM Mg[Cl.
