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Estimating endogenous amino acid flows at the terminal ileum and true ileal amino acid digestibilities in feedstuffs for growing pigs using the
homoarginine method.
Homoarginine (hArg), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) were measured by high-performance liquid chromatography (HPLC).
For FAA analysis, 100-mg samples were first extracted with 7% trichloroacetic acid, containing
homoarginine as an internal standard, then the extracts were washed with diethyl ether to remove trichloroacetic acid (Konosu et al.
In PF and PE, diets and ileal digests, chromic oxide was determined by atomic absorption, total and reactive lysine after reaction of these materials with O-methylisourea to form
homoarginine by high pressure liquid chromatography.
Homoarginine, b-ODAP, and asparagine contents of grass pea landraces cultivated in Turkey.
Evaluation of the furosine and
homoarginine methods for determining reactive lysine in rumen-undegraded protein.
The procedures, including the
homoarginine technique, the feeding enzymehydrolyzed protein and the isotope-dilution technique, are used for estimating the total endogenous losses of AA (Hodgkinson et al., 2003).