However, the substitution Arg377Gln might cause a change in hydrogen bonds to alternative ones with other amino acids, which resulted in the loss of enzyme activity or decreased holoprotein
Rogaev, "Impas 1 possesses endoproteolytic activity against multipass membrane protein substrate cleaving the presenilin 1 holoprotein
," FEBS Letters, vol.
The genetic mutations causing Wilson disease impair the hepatic incorporation of copper into apoceruloplasmin, leading to a reduction in the total serum ceruloplasmin concentration due to the instability of the apoceruloplasmin relative to the holoprotein
with its full complement of copper [24,25].
Herms, "Involvement of presenilin holoprotein
upregulation in calcium dyshomeostasis of Alzheimer's disease," Journal of Cellular and Molecular Medicine, vol.
is a homodimeric protein with a multidomain structure consisting of a) N-terminal extracellular domain (ECD), b) single helical transmembrane region, c) juxtamembrane domain, d) kinase homology domain (KHD), e) linker region, f) GC domain (GCD), and g) C-terminal domain (CTD) (53).
where [DELTA][delta][sup.1]HN and [DELTA][delta][sup.15]N denote the [sup.1]HN and [sup.15]N backbone amide chemical shift change between the apo- and holoprotein
for a particular residue, and [[omega].sub.i] denotes the weight factor of the nucleus which accounts for differences in sensitivity of the [sup.1]HN and [sup.15]N [[omega].sub.NH] = 1.0; [[omega].sub.NH] = 0.154.
To ascertain sites where introduction of amino acid residues would be tolerated without affecting folding of the polypeptides and assembly into holoprotein
, methionine residues were introduced into different regions of the Gy1 gene (Utsumi et al., 1994).
Since ethylene production is overexpressed in the phyB-1 mutant, phyB (holoprotein
) most likely down regulates the genes for ethylene biosynthesis.
of Arabidopsis phytochrome B (phyB) were expressed, reconstituted, and purified as previously described [24, 25].
(13) in which apoproteins are first separated from holoproteins
and then the TC fraction of the holoproteins
is directly measured by ELISA.
Both assays use specific anti-TC antibody rather than imprecise physicochemical methods to separate TC from HC and other holoproteins
. Ulleland et al.
HoloHC was computed as the difference between total Cbl and holoTC and may thus include other minor holoproteins