high-pressure liquid chromatography


Also found in: Encyclopedia.

high-per·for·mance liq·uid chro·ma·tog·ra·phy (HPLC),

a chromatographic technology used to separate and quantitate mixtures of substances in solution. A sample is injected into a moving stream of solvent that flows through a column and detector. Separation during passage through the column occurs by absorption, partition, ion exchange, or size exclusion. The technique is commonly used in laboratories to measure organic compounds including steroid hormones, pesticides and poisons, toxic and carcinogenic compounds, and drugs.

high-per·for·mance liq·uid chro·ma·tog·ra·phy

(HPLC) (hī pĕr-fōr'măns lik'wid krō'mă-tog'ră-fē)
A chromatographic technology used to separate and quantitate mixtures of substances in solution. The technique is used to measure organic compounds, including steroid hormones, pesticides and poisons, toxic and carcinogenic compounds, and drugs.
Synonym(s): high-pressure liquid chromatography.
References in periodicals archive ?
PAH Analysis by High-Pressure Liquid Chromatography (HPLC) with Fluorescence Detector.
The advanced methods for organism identification include high-pressure liquid chromatography and genotypic methods like sequencing of unique 16S rRNA.
(6-9) The present preliminary study was conducted to (1) provide information on the temporal association between fatal peptic ulcer disease presenting as sudden death and NSAID use prior to death, and (2) to examine the diagnostic efficiency of postmortem determination of NSAID levels using high-pressure liquid chromatography (HPLC) on autopsy blood samples.
The American Organization of Agricultural Chemists, for example, recently approved a method that uses an antibody to bind aflatoxin to a matrix in an affinity column, then removes it from the antibody and quantifies, using high-pressure liquid chromatography. This method solved the sample-preparation problem that had caused aflatoxin test results to swing widely, leading to a lot of arbitration between grain buyers and grain sellers.
First, Daly separated the alkaline components from one another using high-pressure liquid chromatography. Then he used mass spectrometry to determine the size of the molecules in the component that made the mouse arch its tail.
Using high-pressure liquid chromatography, USDA-ARS researchers examined the phenolic compounds in leaf, flavedo, albedo and juice vesicle tissues of 35 species of citrus (Rutaceae).
Chitwood's test, which was developed at the ARS Nematology Laboratory in Beltsville, Maryland, is a modification of a method called reversed-phase high-pressure liquid chromatography. With fewer steps than present techniques, the new one takes as little as 30 minutes.
Epidermal retinoid and beta-carotene concentrations were determined by high-pressure liquid chromatography. Topical beta-carotene penetrated well into human and mouse epidermis and induced a 10-fold (human) and a threefold (mouse) increase of epidermal retinyl esters, which demonstrates that topical beta-carotene is converted into retinyl esters by human and mouse epidermis and thus appears as a precursor of epidermal vitamin A.
High-pressure liquid chromatography revealed two or perhaps three proteins within the sample, they report.
Analytical methodologies employing high-pressure liquid chromatography, nuclear magnetic resonance, mass spectrometry and electrophoretic techniques for the assessment of product integrity are being developed by Michel Girard, MCIC.
Chromatographic system for high-pressure liquid chromatography with a diode-matrix UV detector
Comparison of Sebia CAPILLARYS Capillary Electrophoresis with the Primus High-Pressure Liquid Chromatography in the Evaluation of Hemoglobinopathies.

Full browser ?