Moreover, FPG of participants was measured by hexokinase method
, whereas fasting insulin level was measured by chemiluminescent immunoassay; thereafter, homeostatic model assessment of insulin resistance (HOMA-IR) was calculated using the formula: FPG (mg/dL)xfasting insulin ([micro]U/mL)/405 (15).
Plasma glucose was measured by hexokinase method
on ADVIA 1800 chemistry analyzer (Siemens Diagnostics, NY, US).
Plasma glucose was measured by enzymatic hexokinase method
using the ADVIA 1200 Siemens analyzer (Siemens, USA).
Fasting glucose level was determined by using the glucose hexokinase method
(Beckman Coulter AU 2700).
Glucose, BUN, and P4 assays: The plasma samples were obtained in the gray-top tube and sent to the laboratory for glucose analysis by the hexokinase method
(Aekplakorn et al., 2007).
Plasma venous blood glucose levels were estimated using the hexokinase method
on an autoanalyzer (Siemens Healthcare Diagnostics, Inc., West Sacramento, CA 95691 USA).
Fasting blood glucose was determined using the hexokinase method
. Plasma levels of triglyceride, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol were evaluated using an automated chemistry analyzer (Aeroset, Abbott, USA) with commercially available kits (Abbott, USA).
Fasting plasma glucose (FPG) is estimated by hexokinase method
. Total Cholesterol, (TC), HDL-Cholesterol (HDL-C) and triglycerides (TG) were processed in automated analyser, LDL-cholesterol and VLDL were calculated by Friedwald and Fredrickson formula.
Measurement of serum glucose concentration by spectrophotometric technique using hexokinase method
continues to be the gold standard and is used in clinical laboratories.
Donna, "Evaluation of glucose 6 phosphate dehydrogenase from Leuconostoc mesentroides in the hexokinase method
for determining glucose in serum," Clinical Chemistry, vol.
RPG was analysed within 2 hours of sample collection by hexokinase method
while HbA1c was analysed using Tina-quant method on a fully automated chemistry analyser (Roche Modular P800).
Fasting blood sugar (FBS) by Hexokinase method
and free iron by direct iron assay using chromophore ferene[R] were analyzed in blood sample using standard kits in Dimension RxL autoanalyzer, USA and HbAlc by high-performance liquid chromatography method was analyzed in blood sample using Bio-Rad autoanalyzer, USA.