Moreover, FPG of participants was measured by
hexokinase method, whereas fasting insulin level was measured by chemiluminescent immunoassay; thereafter, homeostatic model assessment of insulin resistance (HOMA-IR) was calculated using the formula: FPG (mg/dL)xfasting insulin ([micro]U/mL)/405 (15).
Plasma glucose was measured by
hexokinase method on ADVIA 1800 chemistry analyzer (Siemens Diagnostics, NY, US).
Plasma glucose was measured by enzymatic
hexokinase method using the ADVIA 1200 Siemens analyzer (Siemens, USA).
Fasting glucose level was determined by using the glucose
hexokinase method (Beckman Coulter AU 2700).
Glucose, BUN, and P4 assays: The plasma samples were obtained in the gray-top tube and sent to the laboratory for glucose analysis by the
hexokinase method (Aekplakorn et al., 2007).
Plasma venous blood glucose levels were estimated using the
hexokinase method on an autoanalyzer (Siemens Healthcare Diagnostics, Inc., West Sacramento, CA 95691 USA).
Fasting blood glucose was determined using the
hexokinase method. Plasma levels of triglyceride, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol were evaluated using an automated chemistry analyzer (Aeroset, Abbott, USA) with commercially available kits (Abbott, USA).
Fasting plasma glucose (FPG) is estimated by
hexokinase method. Total Cholesterol, (TC), HDL-Cholesterol (HDL-C) and triglycerides (TG) were processed in automated analyser, LDL-cholesterol and VLDL were calculated by Friedwald and Fredrickson formula.
Measurement of serum glucose concentration by spectrophotometric technique using
hexokinase method continues to be the gold standard and is used in clinical laboratories.
Donna, "Evaluation of glucose 6 phosphate dehydrogenase from Leuconostoc mesentroides in the
hexokinase method for determining glucose in serum," Clinical Chemistry, vol.
RPG was analysed within 2 hours of sample collection by
hexokinase method while HbA1c was analysed using Tina-quant method on a fully automated chemistry analyser (Roche Modular P800).
Fasting blood sugar (FBS) by
Hexokinase method and free iron by direct iron assay using chromophore ferene[R] were analyzed in blood sample using standard kits in Dimension RxL autoanalyzer, USA and HbAlc by high-performance liquid chromatography method was analyzed in blood sample using Bio-Rad autoanalyzer, USA.