The hydroalcoholic and hexane
extracts were transferred to forced air circulation dehydrator (S480AD, Biopar, Porto Alegre--RS, Brazil) at 50[degrees]C, where they were maintained until complete evaporation of the solvent.
00 for hexane
and ethyl acetate extract, depicting that all of the test organism have died.
Lactuca sativa roots treated with distilled water showed the highest mitotic indices, differing statistically from roots subjected to methanol and hexane
extracts with 50, 100, and 200 [micro]g [mL.
Solvents used were methanol, ethanol, hexane
and chloroform-methanol (1: 1).
The difference between hexane
extract and the control was statistically significant with maximum inhibition that exceeds 85% from 100 pi.
The root growth of seedlings in the presence of the AcOEt and hexane
fractions was reduced to null at estimated concentrations of 0.
The crude extract with different solvent extractor (methanol, ethanol, acetone, hexane
, water) was developed using a selected ratio of mobile phase to separate the compound.
Larvicidal activity of leaf extracts of Breyenia vitis-idaea against III instar larvae of mosquito vectors Concentration (ppm) Larval mortality (%) Hexane
Chloroform Aedes aegypti Control 1.
We performed the same procedure with the material collected in Aug/2009 (also leaves and stems), but hexane
(HEX) was used as a non polar solvent instead of DCM, because of its environmental toxicity.
The dry powdered plant material was directly extracted with solvent, however, in two occasions (indicated in the Table) the plant material was first defatted by Soxhlet-extraction with hexane
followed by re-extraction of the marc (Soxhlet) with absolute ethanol; the latter extract assessed for antifungal activity.
In order to evaluate the influence of polymer molar mass, SLE data were obtained using the nonsolvent hexane
, which presented more pronounced anti-solvent effect in the system.
3]) and methanol (MeOH) has led to evaluation of possible substituting mixtures, such as hexane
and isopropanol (iPrOH) (Hara & Radin 1978, Erickson 1993, Gunnlaugsdottir & Ackman 1993, Molina Grima et al.