Ligand-controlled assembly of hexamers
, dihexamers, and linear multihexamer structures by the engineered acylated insulin degludec.
1 [micro]g of RNA was reverse transcribed using the RevertAid First Strand cDNA Synthesis Kit (Thermo) and random hexamers
. Quantitative real-time PCR (qRT-PCR) was performed with SYBR Green PCR Master Mix (Kapa Biosystems, USA).
Two hundred nanograms of total cellular RNA were reverse transcribed (Expand Reverse Transcriptase, Roche) into complementary DNA (cDNA) using random hexamer
primers (Random hexamers
, Roche) at 42[degrees]C for 45 minutes, according to the manufacturer's instructions .
(2008) also reported the absorption enhancing effect of chitosan hexamer
for FD4, and found that its absorption enhancing effect was almost saturable up to 0.5% (w/p) using in situ loop method.
I place "whole" in quotation marks because, as mentioned above, this isn't truly unbiased; however, note that where bias arises from intrinsic sequence features which inhibit replication, or from not-quite-perfect equality of the amount or priming efficiency of each possible random hexamer
, these effects should occur equally in reference and test samples.
The extracted RNA was converted to cDNA using a cDNA synthesis kit (Parstous, Iran) using both oligo(dT) and random hexamer
primers (table 1).
Lobsters, like most crustacean arthropods, have hemocyanin that is composed predominately of hexamer
and dodecamer aggregates, with small concentrations of 24-mers and 48-mers (Mangum et al.
Two hundred nanograms of total RNA was reverse-transcribed into cDNA with random hexamer
primers (Roche Diagnostics, Mannheim, Germany).
Genomic RNA was reverse-transcribed by random hexamer
primers, and 5 [micro]L of the resultant cDNA fragments was then added into a 50 [micro]L PCR reaction system containing 2.5 U TaKaRa LA Taq, 5 [micro]L 10 x LA PCR Buffer II ([Mg.sup.2+] Plus), 1 [micro]L 20 [micro]M former primer PNPF, 1 [micro]L 20 [micro]M reverse transcription- (RT-) primer PNPR, and 8 [micro]L 2.5 mM dNTP mixture.
As is well known, the prediction of protein quaternary structure attributes (such as monomer, dimmer, trimer, tetramer, pentamer, hexamer
, heptamer, and octamer) plays an important role in the structure bioinformatics.
For one, little is known about the mechanisms that promote the incorporation of J chains into polymers that as a result become "pentamers" and how the plasma cell balances its "hexamer
" and "pentamer" output .
cDNA was synthesized from 0.4-0.6 pg RNA, using Superscript II Reverse Transcriptase (Life Technologies) when using random hexamer
primers (for SIX5 mRNA quantification) or using Superscript III Reverse Transcriptase (Life Technologies) when using strand-specific primers (for DMPK sense and antisense mRNA quantification), according to manufacturer's instructions (also see ).