Four different rows of three different microtiter plates were coated in this pattern: wells A1, B1, C1, and D1 were coated with [T.sub.3]; the other eleven wells of each row (A2-A12, B2-B12, C2-C12, and D2-D12) were coated with roasted cashew, egg, seaweed, or latex hevein. Controls and inhibitors were then added as follows: 100 [micro]L of serum diluent to wells A1-A2, B1-B2, C1-C2, and D1-D2; 100 [micro]L of diluent containing 100 [micro]g of [T.sub.3] to wells A3, B3, C3, and D3; and 0.5-120 [micro]g of cashew, egg, seaweed, and latex hevein to, respectively, A4-A12, B4-B12, C4-C12, and D4-D12.
Using this monoclonal antibody against Tg resulted in a 3+ immune reaction with latex hevein (see Table 2).
Lectins have been classified into 12 different families based on their domain structures and phylogenetic analyses: ABA (Agaricus bisporus agglutinin), amaranthin, chitinase-related agglutinin (CRA), cyanovirin, Euonymus europaeus agglutinin (EEA), Galanthus nivalis agglutinin (GNA), hevein
, jacalins, legume lectin, lysin motif (LysM), and nictaba and ricin B families (Jiang et al.
After leaving Apocalyptica in early 2000s, it seems Max unveiled his full potential - besides being a cellist in Finnish National Opera Orchestra, he became member of a multi genre metal band Hevein
and a Finnish folk-pop band Tekija Tuntematon, whose music is based on Finnish poetry.
 Thirteen different hevein
latex proteins have been recognised as allergens by the Allergen Nomenclature Subcommittee of the World Health Organization/International Union of Immunological Societies.
This class of chitinases contains a cysteinerich 40-amino-acid domain at the N-terminus, the chitin binding hevein
domain, a hypervariable domain (which is a proline-rich hinge region), and a catalytic domain .
homologs isolated from the seed of Pharbitis nil L.
Demonstration by mass spectrometry that pseudo-hevien and hevein
have ragged C-terminal sequences.
IgE epitope analysis of the hevein
preprotein; a major latex allergen.
Of note, plant lectins can be divided into 12 different families, including ABA (Agaricus bisporus agglutinin), Amaranthin, CRA (chitinase-related agglutinin), Cyanovirin, EEA (Euonymus europaeus agglutinin), GNA (Galanthus nivalis agglutinin), Hevein
, Jacalins, Legume lectin, LysM (lysin motif), Nictaba and Ricin_B families (Van Damme et al., 2008).
Four major lectin families, namely, the legume lectins, the chitin-binding lectins composed of hevein
domains, the type2 ribosome-inactivating proteins and the monocot mannose-binding lectins comprise the majority of all currently known plant lectins (Van Damme et al., 1998a).