Para determinar la asociacion entre Shh y FLPNS se hizo un analisis de trios caso-progenitores para determinar los alelos, haplotipos y genotipos transmitidos de padres a hijos mediante la tecnica de heteroduplex
Evidence for RAPD heteroduplex
formation in cranberry: implications for pedigree and genetic-relatedness studies and a source of co-dominant RAPD markers.
In fact, we found no evidence of interspecific hybridization using diagnostic COI and ITS-1 markers, even though formation of ITS-1 heteroduplex
bands in hybrids would have facilitated identification of the most likely interspecific hybrids from the C.
resolution using T7 endonuclease I in microbial community analyses.
Materiales y metodos: Se determino la presencia de la mutacion F508del mediante PCR y analisis de heteroduplex
en 110 estudiantes de IV y VII semestre de la Facultad de Medicina.
Quest Diagnostics Incorporated (Madison, NJ), the nation's leading provider of diagnostic testing, information and services, announced that it has entered into a non- exclusive license agreement for the heteroduplex
tracking technology underlying Pathway Diagnostics' SensiTrop HIV co-receptor tropism test.
The genotyping of HCMV strains based on the gB gene have been determined by PCR-based RFLR Single-stranded conformation polymorphism, heteroduplex
mobility analysis, DNA enzyme immunoassay, direct nucleotide sequencing and multiplex-nested PCR (8).
In addition, mutation detection enhancement gels (a particular polyacryl amide specifically designed to enhance the separation of heteroduplex
and single-stranded frangments) can be used to separate a group of fragments with very little difference in length, when standard gelectrophoresis results in only poor separations (Daffonchio et al.
analysis, a method discontinued in 2002, identified four additional mutations found with elevated frequency in African Americans.
Faint bands are believed to be PCR artifacts and are observed in homozygous individuals while heteroduplex
bands only occur in heterozygotes (Rodriguez et al.
After cloning, heteroduplex
molecules may become subject to mismatch repair by the E.
Since the study cohort formed part of a related study on determination of HIV-1 subtypes, status of HIV was confirmed by nested reverse transcriptase-polymerase chain reaction (RT-PCR) and envelope heteroduplex
mobility assay (HMA).