The PAM sequence readout by Arg1333 and Arg1335 of Cas9 facilitates the interaction of +1 phosphate group with the phosphate lock loop that promotes the melting of the local DNA, and gRNA-target DNA heteroduplex
formation in a directional manner .
However, better results with faster rates may not apply to other melting applications that use larger amplicons >100 bp such as heteroduplex
Panel Analysis, a Novel Method for Genetic Identification of Aspergillus Section Flavi Strains.
In this approach, CEL1 cleaves at the site of heteroduplex
indicating mismatches in the sequences.
molecules cause sexing errors in a standard molecular protocol for avian sexing.
The HRM method depends on heteroduplex
formation; therefore, statistically rare homozygous mutations with DNA binding affinity equal to that of wild type might not be detected.
lindheimeri, 4 fragments, 202 base-pair heteroduplex
fragment, 187 base pair fragment from parent of D.
molecules formed between allelic sequences cause nonparental RAPD bands.
The results of the dHPLC showed several heteroduplex
peaks on the chromatograms of samples (Figure 1).
These extra bands are heteroduplex
DNA molecules formed after denaturation and reannealing of species-specific PCR products (i.e., they are not formed in unheated but Hae III-digested mixtures of species-specific PCR products; Fig.
Effect of PCR conditions on the formation of heteroduplex
and single-stranded DNA products in the amplification of bacterial ribosomal DNA spacer regions.