The PAM sequence readout by Arg1333 and Arg1335 of Cas9 facilitates the interaction of +1 phosphate group with the phosphate lock loop that promotes the melting of the local DNA, and gRNA-target DNA
heteroduplex formation in a directional manner [64].
However, better results with faster rates may not apply to other melting applications that use larger amplicons >100 bp such as
heteroduplex scanning.
Heteroduplex Panel Analysis, a Novel Method for Genetic Identification of Aspergillus Section Flavi Strains.
In this approach, CEL1 cleaves at the site of
heteroduplex indicating mismatches in the sequences.
Heteroduplex molecules cause sexing errors in a standard molecular protocol for avian sexing.
The HRM method depends on
heteroduplex formation; therefore, statistically rare homozygous mutations with DNA binding affinity equal to that of wild type might not be detected.
lindheimeri, 4 fragments, 202 base-pair
heteroduplex fragment, 187 base pair fragment from parent of D.
Heteroduplex molecules formed between allelic sequences cause nonparental RAPD bands.
The results of the dHPLC showed several
heteroduplex peaks on the chromatograms of samples (Figure 1).
These extra bands are
heteroduplex DNA molecules formed after denaturation and reannealing of species-specific PCR products (i.e., they are not formed in unheated but Hae III-digested mixtures of species-specific PCR products; Fig.
Effect of PCR conditions on the formation of
heteroduplex and single-stranded DNA products in the amplification of bacterial ribosomal DNA spacer regions.
