Lineage tracing with [sup.3]H-thymidine indicated that these propagating "oval cells" can undergo hepatogenic
differentiation thus participating in the replenishment of liver parenchyma.
(29) The current investigation also showed that HWJMSCs expressed early- and mid-liver-specific genes, such as CK19, CK18, albumin, and AFP when they were cultured for long-term without any hepatogenic
Cells at the density of 1.5 x [10.sup.4]/[cm.sup.2] were serum deprived for 2 days, then exposed into hepatogenic
media cantaining 5 cc low glucose DMEM supplemented with 1 [micro]mol/ml dexamethasone, 20 ng/ml HGF, 20 ng/ml FGF4, [10.sup.-4] M ascorbic acid 2-phosphate, 100 mg/ml streptomycin, 1 mM sodium butyrate, and 1 [micro]M RA (all from Sigma-Aldrich, USA) for a duration of 28 days; cell media were replaced twice weekly.
However, other reports demonstrated that hepatogenic
differentiation after transplantation of BMSCs occurred at a low frequency in a model of acute liver injury [18, 36].
Rafalowska, "Enhanced potassium-stimulated y-aminobutyric acid release by astrocytes derived from rats with early hepatogenic
encephalopathy," Journal of Neurochemistry, vol.
With this nanoporous coculture membrane, the mRNA expression of hepatogenic
genes could be induced in hASCs due to the presence of mouse hepatocytes.
When exposed to hepatogenic
factors and cytokines, hSKPs can cross the lineage border and generate hepatocyte-like cells .
Caption: FIGURE 5: Hepatogenic
differentiation of transplanted hPMSCs in rat livers (20x).
and chondrogenic differentiations showed morphologic changes of ASCs into the polygonal/flattened and rounder/cuboidal shaped cells, respectively.
When exposed to in vitro hepatogenic
differentiation, ADHLSCs are capable to differentiate, either in vitro or in vivo, into hepatocyte-like cells .
differentiation of stem cells has created trust and promise for use of these cells in hepatic tissue engineering and liver replacement.
As a result stem cells could attenuate ALI and transduce hepatic recovery either incubated or not with hepatogenic