However, for the liver of HFD rats, the hepatic cords
appear to were be arranged disorderly.
In the liver, there was centrilobular and mid-zonal hemorrhage with rare hepatocellular necrosis and atrophy of hepatic cords
, and perivenular fibrosis.
After the ischemic/reperfusion injury, the hepatocytes are swollen and the hepatic cords
are disorganized (arrow in C).
The entrapped hepatic cords
were negative for AE1/AE3 (f) (arrowhead), but AE1/AE3 highlighted the entrapped bile ducts (f) (arrow) (immunoperoxidase; original magnification: x200).
Inset shows prominent hepatocellular necrosis and dropout, and erythrocytes replace the hepatic cords
(there is some artifactual formalin pigment [acid hematin] in necrotic hepatocytes).
Many hepatocytes showed accumulation of lipoid material while few areas showed derangement of hepatic cords
and necrotic changes of hepatocytes with occasional basophilia of nucleus and vacuolar cytoplasmic changes in hepatocytes.
As shown in Figure 3(a), clear structure and regular lobular were observed, where hepatic cords
remained legible and radiated from central vain in NC group.
These cells were located in hepatic cords
and showed a tendency to form bile canaliculi.
It was found that under high dose of cadmium chloride (0.0004 g Cd/0.04 ml) the rigorous degenerative alterations in the hepatic cords
and disruption in the sequence of cells around the blood vessels was observed in liver of Larus argentatus.
Mean liver aspartate aminotransferase at the end of the experiment in groups A, B and C were 97.8819.45, 148.2516.48 and 96.1317.95U/L, while alanine transaminase levels were 51.5015.38, 96.7510.91 and 49.6312.08 U/L (pless than 0.05 each) On histological examination of group B, the normal hepatic architecture was distorted with loss of classically arranged hepatic cords
. Vascular congestion was present with centrilobular necrosis, marked by pyknotic nuclei and vacuoles.
The dose limiting side effects were seen in kidney but repeated higher doses of cisplatin cause massive hepatic toxicity including dissolution of hepatic cords
, focal inflammatory lesions & necrosis.
Kupffer cells were not significantly increased when all the gro-ups were compared with p-value less than 0.06; similar chan-ges in the liver lobules of animals treated with fluoride were reported earlier also.24,25 Current findings also corroborate with those of the earlier studies in which necrosis of hepatocytes, pyknotic nuclei and deranged hepatic cords
were reported in fluoride-treated adult rats.16,20,24 Finding of current investigation are also comparable with an earlier study in which rabbits were treated with fluoride at a dose of 10 mg/kg for 15 weeks; focal necrosis of hepatocytes with hyperplasia at places was observed, however, no significant incre-ase in the number of Kupffer cells was reported.26