solution irrigation throughout the procedure kept the tissue moist.
7 L of heparinised
saline (2500 IU/L unfractionated sodium heparin) or with heparin-free saline to assess the effect of heparin priming.
Patient was heparinised
with 300 U/Kg BW of Heparin.
Whole blood (20ml) was collected by jugular vein puncture into mineral free heparinised
The right carotid artery was cannulated with heparinised
cannula and then connected to pressure transducer (Gould Statham P231D, USA) for assessment of blood pressure [systolic arterial pressure (SAP), mean arterial pressure (MAP), and diastolic arterial pressure (DAP)] and heart rate (HR).
blood from healthy volunteers was used for the isolation of Polymorphonuclear cells (PMNs).
The circulating nurse gathers equipment as per the recommended best practice list and prepares bags of heparinised
saline for irrigation and flushing of catheters and wires during the procedure.
The first two sets were collected in heparinised
At the end of the treatment period, the overnight fasted (water allowed) animals were anaesthetized, and blood samples were collected by retroorbital puncture in heparinised
(for haematological) and nonheparinised tubes for biochemical analysis (Semi Auto Analyzer, RA 50, Bayer) and serum electrolytes (Electrolyte Analyzer, EC lyte Transasia) .
Osteocalcin in heparinised
plasma was measured by a solid-phase two-site chemiluminescent enzyme-labelled immunometric assay (Immulite Osteocalcin, Diagnostic Product Corporation, Los Angeles, California).
After sterilisation procedures with alcohol, the distal portion of the animal's tail was sectioned with a little prick and 25 ml of blood were collected after each 3 minutes stage over the incremental test in heparinised
micro capillary tubes.
After the determination of blood glucose and TGs, the rats were euthanized by an intraperitoneal injection of sodium pentobarbitone (200 mg kg-1; Euthanaze, Centaur Labs, South Africa) and 5 mL of blood was obtained by cardiac puncture and placed into heparinised