heparin lyase

(redirected from heparinase)

hep·a·rin ly·ase

an enzyme eliminating Δ-4,5-d-glucuronate residues from heparin and similar 1,4-linked polyglucuronates.
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A second sample was obtained towards the end of surgery, with the patient still on BPC and upon start of rewarming at 32[degrees]; this sample was processed in a different 360 [micro]L tray with heparinase, again with kaolin added.
The TEG 6s global-hemostasis cartridge includes the traditional contact-activation kaolin test--the functional fibrinogen test--to access the contribution of fibrin to the overall clot strength, and the RapidTEG (rTEG) for faster results, with heparinase kaolin used to assess the effect of heparin on coagulation.
Thus, dermatan sulfate was removed from isolated GAGs samples using depolymerisation with chondroitinase B (from Flavobacterium heparinum); then, the mixture of heparinase I and heparinase III (both from Flavobacterium heparinum) was added to a reaction medium in order to remove heparin sulfate/heparin.
In such situation, heparin assay should be done or plasma sample should be treated with heparinase and then the test should be repeated.
21 The synergistic microbial activity results in production of various exotoxins and enzymes like collagenase, heparinase, hyaluronidase, streptokinase, and streptodornase, which lead to tissue destruction and spread of infection.
Specific modifications include the addition of heparinase to the intrinsic test, which allows for assessment of enzymatic factors and for the effects of certain coagulation-altering drugs (e.
The development of sensitive miRNA-detection assays that are based on other PCR-free technologies (5) or the use of heparinase may circumvent this current limitation (3).
A commercially available heparinase TEG revealed an increased maximum amplitude of 67 mm (normal value: 42-65 mm), reflecting a hypercoagulable state.
Makes barrier function of endothelium stronger by substituting with endogenous glycosaminoglycans inhibited by heparinase, binding to endothelial cells
The results of an international clinical trial led by Duke University researchers in 2005 confirmed that heparinase is not a suitable replacement for protamine, and the compound was discontinued from development.
The CFT is used as a dosage guide for fresh-frozen plasma, the MCF to monitor platelet infusion or fibrinogen requirements, heparinase (Heptem) to calculate protamine dosage, and the addition of aprotinin to assess the need for antifibrinolytic therapy.
The effects of heparin, protamine, and heparinase 1 on platelets in vitro using whole blood flow cytometry.
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