was permitted to react with [H.sub.2][O.sub.2] along with GSH, then the remainder of GSH was permitted to react with DTNB.
Blood Sample Collection and Hemolysate
Preparation: Leaf-nosed viper (Eristicophis macmahonii) specimens were collected from Nushki, Balochistan, Pakistan.
The Hg and Se incorporated to these substances were found in stroma-free hemolysate
but not in plasma.
Next, 1.5 mL of the blood sample was anticoagulated with EDTA for the preparation of erythrocyte hemolysate
in order to determine the effects of DZN on the activities of antioxidant enzymes and 3.5 mL of the blood sample was transferred into plane tubes and centrifuged for 10 minutes at 1800Xg for serum separation in order to measure malondialdehyde (MDA), total thiol molecules, and total antioxidant capacity (TAC) as biomarkers of oxidative stress in blood serum.
According to the company, the SEKURE HbA1c assay is used in clinical laboratories to measure the percent concentration of HbA1c (NGSP) or the HbA1c fraction mmol/mol (IFCC) in human venous whole blood and hemolysate
. It is an enzymatic assay which is NGSP certified and utilizes an onboard pretreatment step on Sekisui Diagnostics SK 500 Clinical Chemistry System, eliminating time-consuming manual preparation prior to testing.
After another centrifugation at 15,000 xg for 45 min, the hemolysate
was separated into aliquots and stored at -80[degrees]C until use.
Compared to microglia, there are few reports concerning the effects of isoflavones on astrocytes; genistein suppresses neuroinflammatory changes induced by hemolysate
 or amyloid [beta] .
The pH of the hemolysate
was adjusted to 8.7 with a solid Tris base, and applied to the prepared Sepharose 4B-L-tyrosine-sulfonamide affinity column equilibrated with 25 mM Tris-HCl/22 mM Na2SO4 (pH 8.7).
Nuriman, "Hypocholesterolemia is associated negatively with hemolysate
lipid peroxidation in sickle cell anemia patients," Clinical and Experimental Medicine, vol.
Plasma and Hemolysate
. After decapitation, blood samples were collected into commercial tubes with Li-Heparin as an anticoagulant.
Erythrocytes catalase (EC 184.108.40.206) activity was estimated in the hemolysate
following the method of Greenwald (1985)  based on the scavenging of hydrogen peroxide (0.019 M) in the presence of phosphate buffer (0.1 M, pH 7.4) by catalase present in the sample.