Lastly, they transplanted the genetically engineered hemogenic
endothelial cells into mice.
Particularly, it regulates the emergence of the HSPC population from hemogenic endothelial cells of the aorta-gonad-mesonephros (AGM) during embryonic, definitive hematopoiesis [74, 75], multiple stages of lymphocytic differentiation , and LT-HSC self-renewal and quiescence in the bone marrow niche .
The sheer complexity and density of these hemogenic transcriptional networks limit a precise understanding of MTG16 function in HSPC emergence, but the overall effect of MTG16 knockout in zebrafish is a severe reduction in definitive hematopoiesis .
Schmitt et al., "Repression of arterial genes in hemogenic endothelium is sufficient for haematopoietic fate acquisition," Nature Communications, vol.
The hemogenic endothelial phenotype was defined as [KDR.sup.+][CD117.sup.+][CD45.sup.-] cells [102-104] and demonstrated that these cells give rise to multilinear hematopoietic progenitors which are [KDR.sup.-][CD117.sup.+][CD45.sup.+] cells and can be distinguished from mature blood cell types having the [KDR.sup.-][CD31.sup.[+ or -]][CD45.sup.+].
In summary, the results of the differentiation show that the cells, obtained in greater quantity on day 8 of differentiation, are hematopoietic progenitor cells coming from a hemogenic endothelium.
However, its transient activation was also detected in hemopoietic progenitor populations called "hemogenic endothelium" [17-19].
However, transient activation of Pvec was also found in hemogenic endothelium, a progenitor population that differentiated to endothelial and definitive hemopoietic cells lineages .
Citation: " Induction of a Hemogenic
Program in Mouse Fibroblasts"; Carlos-Filipc Pcrcira et al.; Cell Stem Cell, 2013; DOI: 10.1016/j.stem.2013.05.024