The hemizona assay and induced acrosome reaction test are valuable predictors of IVF outcome [7, 9, 10].
Hemizona Assay. After incubation of sperm samples for 2 h, sperm-zona interactions were assessed using our hemizona assay, as described previously  but with modifications.
Presently available functional tests under these categories include, zona-free hamster oocyte sperm penetration test (22), hemizona assay (HZA) (23) and the hypoosmotic swelling (HOS) test (24).
Hemizona assay has an excellent predictive power for IVF outcome and for the identification of male infertility but it requires technical expertise to use expensive micromanipulators to bisect native human oocyte, which are difficult to obtain owing ethical restrictions.