hemizona assay

hem·i·zo·na as·say

diagnostic test evaluating the binding capacity of a sperm to the zona pellucida.

hemizona assay

an in vitro test of sperm function in which a human zona pellucida is divided in half and one half is incubated with sperm from a donor known to be normal and the other half with sperm from the patient being tested. The number of sperm bound to each half is calculated, and that from the patient's sperm is divided by that from the donor's sperm. A figure of less than 0.60 indicates abnormal patient sperm.
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The hemizona assay and induced acrosome reaction test are valuable predictors of IVF outcome [7, 9, 10].
The number of spermatozoa tightly bound to the zona was counted; the results of the hemizona assay were expressed as the hemizona assay index: the ratio of the number of spermatozoa bound to the test droplet to that of spermatozoa bound to the control droplet.
0 Table 2: Sperm motion characteristics observed through computer-assisted semen analysis and hemizona assay results of the washed spermatozoa after 2 h of incubation.
Presently available functional tests under these categories include, zona-free hamster oocyte sperm penetration test (22), hemizona assay (HZA) (23) and the hypoosmotic swelling (HOS) test (24).
Hemizona assay has an excellent predictive power for IVF outcome and for the identification of male infertility but it requires technical expertise to use expensive micromanipulators to bisect native human oocyte, which are difficult to obtain owing ethical restrictions.