For hanging drop
cultures, all tissue pieces from each gonad were pooled.
Total RNA was extracted from cells, hanging drops
, or cell sheets using the RNeasy Mini Kit (Qiagen, Valencia, CA).
The use of the hanging drop
technique means that one does not need an inverted phase contrast microscope to see the cells, i.e.
phaseolina was present for all seed treatments in which the soil was infested with cornmeal sand inoculum or when the hanging drop
infestation method was used (Table 2).
If, for example, a diphtheroid is isolated from a spinal fluid specimen, it would be important to rule out Listeria with a hanging drop
To obtain mixed aggregates, DP cells or LF, labeled by RFP, and KCs were mixed in 1 : 1 proportion and cultured in a hanging drop
in DMEM medium containing 4 mM glutamine and 10% FBS at a concentration of 7 x [10.sup.3] cells per aggregate.
The scaffold-free platforms market is further sub-segmented into low attachment surfaces, and hanging drop
The most rapid presumptive method to identify the presence of vibrio, in the stool sample is to observe for darting type of motility in the hanging drop
preparation of the stool; but culture is must as culture consider as gold standard method for identification.