In the studies conducted, it was shown that outer hairy cells
in cisplatin cochlea, spiral ganglion cells (SGC), and cochlea basal and mid-turn parts in cochlea and Reissner's membrane and stria vascularis are affected [3, 7, 8].
leukemia (HCL) is an uncommon haematological malignancy characterized by clonal disease of mature B lymphocytes with fine villous cytoplasmic projections (hence the name hairy cells
) found in the bone marrow and peripheral blood.
As shown in Figure 2, double staining allows resolution of single B cells expressing CD103 and separates normal CD103-negative B cells from CD103 positive B cells (hairy cells
) in the clot section containing mostly peripheral blood elements (a small population of HCL was detected by flow cytometry).
A blood smear showed 10% hairy cells
, and a bone marrow biopsy confirmed a diagnosis of hairy cell
leukemia and interstitial infiltration of CD20-positive, monoclonal antibody DBA.44-positive, and tartrate-resistant acid phosphatase-positive cells.
An energetic exhaustion of the hairy cells
is assumed as the physiological mechanism which causes the temporary threshold shift (TTS) as the first step towards a permanent hearing deficiency (permanent threshold shift, PTS).
Although the etiology of paraneoplastic vasculitis is not yet clearly known, suggested mechanisms are: (i) vascular damage caused by cross-reactions between antibodies against tumor cells and the vascular endothelium (ii) vascular inflammation caused by immune complexes involving tumor antigens (iii) direct vascular damage caused by the hairy cells
in hairy cell
Lysates of hairy cells
, platelets, and red blood cells (RBCs) were prepared to test the specificity of 14G6 for type-5 TRAP by immunoprecipitation.
are observed in the peripheral blood in 90% of HCL patients.
The target regions that are mainly affected by cell damage mechanisms are the spiral ganglion cells, the lateral wall and the hairy cells
in the cortical organ .
Typical hairy cells
(Figure 1, B) have round to reniform nuclei without prominent nucleoli and with a moderate amount of pale cytoplasm.
Table 2 Selected flow cytometry panels and their objectives Antibody panel Objective CD5, CD19 CD10 Detects chronic lymphocytic leukemia CD20 CD11c, CD22 Detects B-cells or hairy cells
CD33, CD13, HLADr Resolves myeloid leukemia cells CD7, CD1, CD2 Defines mature and immature T-cells subsets CD38, CD56, CD45 Resolves plasma and myeloma cells CD5 CD14 CD19 Defines T and B cells and monocytes in cerebral spinal fluid CD16 CD13, HLADr Defines acute promyelomonocytic leukemia CD71, CD3, CD4 Activation of T-helper/inducer cell subset CD20, CD79, CD22 Evaluation of CD79 to resolve certain B-cell malignancies CD33, UIC2, CD34 Evaluate drug resistance or progenitor cells Source: Roswell Park Cancer Institute
Serum band 5 tartrate-resistant acid phosphatase (TrACP; EC 126.96.36.199)  is ordinarily expressed in certain differentiated cells of the mononuclear phagocyte system, notably osteoclasts and alveolar macrophages , and pathologically in Gaucher's cells  and in the hairy cells
of leukemia reticuloendotheliosis .