Thirty haemoglobinuric buffaloes were selected from district Chakwal during December 2010 - January 2011.
Serum samples of haemoglobinuric and healthy buffaloes were analyzed through spectrophotometer using diagnostic kits as per manufacturer's recommendations for determination of glucose, cholesterol, alkaline phosphatase, bilirubin (total and direct), calcium and phosphorous.
Thirty haemoglobinuric buffaloes were divided into three groups each comprising of ten animals for allocation of respective treatment packages as described below
Data of haemoglobinuric and healthy buffaloes with respect to haematological and biochemical values were analyzed by applying independent sample t-test whereas; treatmentpackages were compared with respect to their recovery rates and average number of doses utilized by applying Chi-square test for proportion and Kruskal Wallis H test respectively at 5% significance level using SPSS 17 software.
39 mg /dl) were recorded in haemoglobinuric buffaloes.
Haematological and biochemical values (Mean+-SD) of haemoglobinuric and healthy buffaloes,
05) difference was recorded between haemoglobinuric and healthy buffaloes with respect to mean corpuscular haemoglobin concentration and serum copper level with an odds ratio of 0.
Thirty haemoglobinuric buffaloes were selected from district Chakwal during the months of December, 2010 and January, 2011.
Serum biochemical analysis: Serum samples of haemoglobinuric and healthy buffaloes were analyzed for determination of phosphorous, copper, molybdenum and selenium.
The findings with respect to haematological parameters of haemoglobinuric animals are in agreement with Dalir- Naghadeh et al.
79mg/dl) in haemoglobinuric buffaloes and odds ratio of 7.
18 Table 4: Major clinical finding of haemoglobinuric
buffaloes Major clinical Recorded (%) Red/coffee colored urine 100 Straining/ Constipation 100 Pale Mucous Membrane 100 Temperature Subnormal-60, normal-20, elevated-20 Rumen motility Suspended-70, normal-30 Frequent urination 80 Anorexia 70 Drop In milk production 80 Recumbency 70 Table 5: Urinalysis of haemoglobinuric