Following trypsinization, Trypan blue (0.1% v/v) was added to the cell culture well (1:1, 5 min) prior to cell counting (stained and unstained) using a haemocytometer
. The amount of live (Trypan blue negative) and dead (Trypan blue positive) cells was expressed as the number of stained or unstained per ml.
The number of cell were counted at the end of treatment using haemocytometer
. The culture aliquot were pelleted in 50mL falcon tube via centrifugation (HITACHI high-speed refrigerated centrifuge CR22N) at 15000xg for 15min at 4[degrees]C.
Leukocytes (WBCs) count 103 /mm3 was calculated by Haemocytometer
method (Kolmer et al., 1986).
Samples are taken with a sterile pipette, dropped about 0.1-0.5 mL on haemocytometer
, and then observed through a microscope .
Bone-marrow cells were collected from both femurs of individual naive mice, washed, counted in a haemocytometer
, seeded at [10.sup.6] in 1mL of RPMI 1640 medium, 10% FCS, and rmIL-5 (1ng/mL; optimal concentration, as previously defined ) in 48-well clusters, and incubated at 37[degrees]C, 5% C[O.sub.2]/95% air, for 7 days.
The cells were counted using a haemocytometer
, and their viability (~90%) was determined by 0.4% trypan blue dye exclusion.
Haemogram (CBC: Complete blood count, Full blood count, FBE: Full blood exam) is one of the most commonly requested screening test both in adults and to determine certain types of blood disorders and also it provides valuable information about patients diagnosis, prognosis and response to treatment.2 Traditionally the blood counts were performed manually using the haemocytometer
. The high workload experienced by many haematology laboratories coupled with the reduction in staff leads to the introduction of automated haematology analysers where it was not possible to screen each slide under the microscope.
The cells were adjusted to 3 x106 cells/ml with the dilution of normal saline and counting by using haemocytometer
. The viability of tumor cells were observed by trypan blue dye (0.4%) exclusion assay.
Leucocyte counts: Total leucocytes count (TLC) was done following standard haematological techniques using haemocytometer
. A thin smear was drawn from each blood sample on grease free glass slide, fixed in methanol and stained for 40 minutes in Giemsa solution and observed under microscope using oil immersion (100X) objective.
Counting was then done using the counting chamber in the haemocytometer
The blood cell count before and after incubation of these microspheres with blood was manually counted using a haemocytometer
to study any adhesion of cells onto the microspheres.
The conidial concentration of the suspensions was determined using a haemocytometer