After incubation, the slides were quickly rinsed with SA solution, after which haemo- lymphocytes adhering to the glass slide were fixed by immersing the slide(s) in methanol for at least 5 minutes and thereafter stained with Wright-Giemsa stain.
hupensis haemo- lymphocytes; this stain clearly differentiated nucleus from the cytoplasm.
Through this method, the haemo- lymphocytes nucleus also could be separated into three size groups: large, medium, and small, with diameter ranges of 8.1-8.6 um, 5.5-6.0 um, and 4.2- 4.7 um, respectively.
Table I.- Three size groups of nucleus of haemo- lymphocytes of Oncomelania hupensis.
Through TEM examination, haemo- lymphocytes appeared round and containing a round section shaped nucleus surrounded by cytoplasm (Fig.
(2012) who had previously separated haemo- lymphocytes (lymphocytes) of O.
However, in agreement with the amoeboid-shaped haemo- lymphocytes observed in the present study, previously Sminia and Barendsen (1980) had named haemo-lymphocytes as amoebocytes in three snail species, L.
hupensis snail: thus, confirming the mechanism of haemo- lymphocytes action against foreign particles or parasites.
hupensis already infected with the fish trematode, Exorchis sp., suggesting that the innate immunity response and haemo- lymphocytes of O.