The patient was treated with standard dosage intravenous haem arginate (Normosang), a formulation of
haematin, for 6 days.
The stain haematoxylin is not a dye, but develops colouring properties on oxidation to
haematin. This substance
haematin has little or no affinity for tissue elements and requires an inorganic ion to act as a "go between" (called a mordant) between the dye and the tissue.
(24) In treatment of malaria, CQ binds to the
haematin (a toxic by product of hemoglobin proteolysis containing iron as a center) and prevents its incorporation in to haemozoin.
During this process a build up of
haematin occurs which is potentially toxic to the parasite (Fig.
Blood (0.1 ml) was added to N/10 HCl that converts Hb into acid
haematin. Brown color of the
haematin was matched with brown color of the comparator and calculation was done as described in the method.
