The patient was treated with standard dosage intravenous haem arginate (Normosang), a formulation of haematin
, for 6 days.
The stain haematoxylin is not a dye, but develops colouring properties on oxidation to haematin
. This substance haematin
has little or no affinity for tissue elements and requires an inorganic ion to act as a "go between" (called a mordant) between the dye and the tissue.
(24) In treatment of malaria, CQ binds to the haematin
(a toxic by product of hemoglobin proteolysis containing iron as a center) and prevents its incorporation in to haemozoin.
During this process a build up of haematin
occurs which is potentially toxic to the parasite (Fig.
Blood (0.1 ml) was added to N/10 HCl that converts Hb into acid haematin
. Brown color of the haematin
was matched with brown color of the comparator and calculation was done as described in the method.