antibody assay: Haemagglutination
antibody (HA) assay was used for the evaluation of effect of herbal drugs on humoral immune response of rabbits against SRBCs (Ranjithet al.
Serum samples were analysed using beta (P) micro haemagglutination
inhibition technique to determine the antibody titre levels to Newcastle disease vaccinations as a measure of the immunological response elicited in the experimental birds.
Guidelines from the United States of America (USA) and Europe recommend a combination of two tests: the first one is a non treponemal (cardiolipin, reaginic) test, essentially Venereal Disease Research Laboratory (VDRL) or rapid plasma reagin (RPR); and the second is a treponemal test, essentially TP haemagglutination
assay (TPHA), TP particle agglutination, or the fluorescent treponemal antibody absorption (FTA-abs) test (3,4).
An improved haemagglutination
test for study of canine parvovirus.
They were confirmed by hydatid haemagglutination
test and FNAC.
inhibition titre (log 2) of broilers at 12, 24, and 35 d of age are presented in Figure 1.
Specific treponemal antibody tests such as treponemal enzyme immunoassay (EIA), Treponema pallidum haemagglutination
(TPHA) and fluorescent antibody absorption (FTA-abs) tests have higher sensitivity and specificity than the nontreponemal tests and are used as confirmatory tests for syphilis after a reactive nontreponemal test has been reported1-4.
The role of Casoni's skin test and indirect haemagglutination
test in the diagnosis of hydatid disease.
The effects of the combination were compared to several positive controls (acyclovir, ribavirin and amantadine hydrochloride) and assessment of the effects was done by standard viral viability tests (plaque reduction assay, cytopathogenic assays, virus titrations, analysis of the viral proteins in virus-specific enzyme immunoassays, and haemagglutination
Routine laboratory tests were normal and the indirect haemagglutination
test was found to be positive.
2mg 2/5 3/5 60 Positive - 5/5 0/5 0 Control Negative - 0/5 5/5 100 Control Toxicity 20gm 0/5 5/5 100 Control Key: D = No of dead mice N = No of mice inoculated MHA = Median haemagglutination
titre Table 3: Inhibition of yellow fever virus replication in chick embryo by aqueous extract of Gynostemma pentaphyllum Inoculation Extract Egg MHA litre % regimen concentra- mortality (Log 2 inhibition controls tion (D/N) reciprocal) (mg/mL) allantoic Pre 200 0/5 1.
Data included urinalysis, blood count, indirect haemagglutination
test (IHA), abdominal ultrasonography (AUS), intravenous urography (IVU) and computerized tomography (CT).