8,9) Positive granzyme
B and perforin expression and germline configuration of the TCR in ANKL are features that are helpful in distinguishing these 2 entities.
6,13) A subset of cases exhibits positivity for the cytotoxic-associated antigens such as T-cell intercellular antigen 1 (63%) and granzyme
They also express CD303 (blood dendritic cell antigen 2, BDCA2), cutaneous lymphocyte-associated antigen (CLA), granzyme
B, and TCL1.
and are positive for such cytotoxic markers as TIA-1, granzyme
, and perforin (Figure 3).
with expression of cytotoxic markers such as TIA1, perforin, and granzyme
B (Figure 3, C and D).
Most cases express cytotoxic granule-associated proteins such as TIA1, granzyme
, and/or perforin.
Anaplastic large cell lymphoma is predominantly an activated CD4+ T-cell tumor with an unusual cytotoxic TIA-1+, granzyme
B+, perforin+, granulysin+, EMA+, punctate cytoplasmic clusterin+ phenotype (93,96-101) (Figure 1, D and E).
2,18) A significant proportion of cases exhibit expression of at least one cytotoxic marker (TIA1, granzyme
B, and perforin), and Epstein-Barr virus is reliably negative.
The antibodies used in this study included ALK, BCL6, CD21, CD30, CD68, CD45, granzyme
B, [kappa] light chain, and [lambda] light chain (Dako), CD3, CD4, CD5, CD8, CD10, CD20, CD23, CD43, CD56, PAX5, MPO, TdT (Novocastra, Newcastle, United Kingdom), BCL2 (Zymed, South San Francisco, California), CD2 (US Biological, Swampscott, Massachusetts), and TIA-1 (Coulter/Immunotech, Westbrook, Maine).
B+, TdT-; most cases show clonal TCR gene rearrangements.
The expression of cytotoxic proteins, TIA-1, perforin, and granzyme
B is also variable.
In case 3, the neoplastic cells were also positive for CD4, CD43 (Figure 4), EMA, and granzyme