gradient elution

gra·di·ent e·lu·tion

elution in column chromatography in which a changing pH or ionic strength is used to separate substances.
Farlex Partner Medical Dictionary © Farlex 2012
References in periodicals archive ?
In the findings of a published study by Geng in the Journal of Science China Chemistry on the two variable-theory of peptides under gradient elution in 2017, a dynamic separation method for the rapid identification and improvement of the selectivity of peptides achieved by simply changing the mobile phase flow-rate (MPF-R).
Initially, a gradient run from 2% to 92% was achieved to determine whether isocratic or gradient elution was appropriate [9].
speciosum extract from three different batches was performed using a rapid high-performance liquid chromatography method under the conditions using gradient elution with a mobile phase of acetonitrile-water, as previous described by our group [8].
The water (containing 0.1% formic acid and solvent A) and acetonitrile (solvent B) was used as mobile phase with gradient elution. The gradient was as follows: 0 min 5% B, 0.5 min 5% B, 2.0 min 80% B, 2.1 min 5% B, and 3.0 min 5% B, and then stopped.
The four synthetic food dyes are efficiently separated using an optimized gradient elution in a single run within 15.1 min.
Gradient elution was performed with (A) 80% methanol and (B) 20% water (pH 3.5 modified by o-phosphoric acid) at flow rate of 1.0 mL min-1 after filtration through 0.45 um pore size.
The fractionation of the ethyl acetate extract was done through a series of gradient elution normal phase gravity column chromatography (GCC) on a 60 - 230 mesh silica gel stationary phase [11].
Recommendations are made concerning initial column selection and the choice of isocratic or gradient elution approaches.
The separation was performed on ODS-C18 column (100 x 4.6 mm, 3 [micro]m) with gradient elution by the mobile phase A, methanol-0.1% formic acid (10 : 90), and mobile phase B, methanol-acetonitrile-0.1% formic acid (50:30:20); the gradient elution processes is 0min (0% B), 15 min (8% B), 45 min (30% B), 60 min (35% B), and 75 min (100% B); the flow rate was 1.0mL/min; the column temperature was 40[degrees]C; and the detection wave length was set at 280 nm.