gradient elution

gra·di·ent e·lu·tion

elution in column chromatography in which a changing pH or ionic strength is used to separate substances.
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6 x 250 mm, 5 [micro]m; Agilent) with gradient elution (at the rate of 1.
Contract notice: Supply liquid chromatograph with gradient elution, equipped with refractive index detector, uv detector, automatic injection of sample, communication with different parts
Featured equipment includes two custom designed FR loops, ICP and gradient elution ion chromatography analyzers, FTIR, DCA Goniometer, Parr pressure reactors, UV-VIS scanning spectrophotometers, various digital viscometers, and advanced proprietary geochemical modeling software.
Table 1: Gradient elution method performed with binary solvent system using as mobile phase.
The majority of published methods for quantifying iohexol have used capillary electrophoresis or gradient elution liquid chromatography (LC) coupled with ultraviolet (UV) detection.
The programme of gradient elution was identical to that described in identification and quantification of phospholipid classes, but carried out at a flow rate of 3.
High-performance liquid chromatography cannot separate some samples within the usual fixed, or isocratic, conditions, but requires gradient elution, or solvent programming, which is a change in mobile phase composition during the separation in order to progressively reduce sample retention.
Running a gradient elution too fast compromises resolution and can lead to incorrect analyses.
They are normally used in the gradient elution mode because they allow one to start with low content of organic component in the eluent (ACN or THF) then the strongly retained components will move faster for improved resolution.
To determine the loading capacity at which Sample Self Displacement occurs, initial fractions of the gradient elution peak of Isis Pharmaceuticals' ISIS 2302 (Alicaforsen) were analyzed.