Protein retention time predictions in the gradient elution
mode were based on experimental chromatographic runs.
6 x 250 mm, 5 [micro]m; Agilent) with gradient elution
(at the rate of 1.
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Featured equipment includes two custom designed FR loops, ICP and gradient elution
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Table 1: Gradient elution
method performed with binary solvent system using as mobile phase.
The majority of published methods for quantifying iohexol have used capillary electrophoresis or gradient elution
liquid chromatography (LC) coupled with ultraviolet (UV) detection.
The programme of gradient elution
was identical to that described in identification and quantification of phospholipid classes, but carried out at a flow rate of 3.
A gradient elution
from hexane to 25% ethyl acetate in
High-performance liquid chromatography cannot separate some samples within the usual fixed, or isocratic, conditions, but requires gradient elution
, or solvent programming, which is a change in mobile phase composition during the separation in order to progressively reduce sample retention.
Running a gradient elution
too fast compromises resolution and can lead to incorrect analyses.
They are normally used in the gradient elution
mode because they allow one to start with low content of organic component in the eluent (ACN or THF) then the strongly retained components will move faster for improved resolution.
To determine the loading capacity at which Sample Self Displacement occurs, initial fractions of the gradient elution
peak of Isis Pharmaceuticals' ISIS 2302 (Alicaforsen) were analyzed.