gradient elution

gra·di·ent e·lu·tion

elution in column chromatography in which a changing pH or ionic strength is used to separate substances.
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Gradient elution was performed with (A) 80% methanol and (B) 20% water (pH 3.
Liquid Chromatographic Method for Simultaneous Determination of Citalopram with NSAIDs in Bulk Drug, Pharmaceutical Formulation and Human Serum
The fractionation of the ethyl acetate extract was done through a series of gradient elution normal phase gravity column chromatography (GCC) on a 60 - 230 mesh silica gel stationary phase [11].
TOXICITY, ANTIMICROBIAL, AND ANTIMUTAGENCICITY POTENTIALS OF THE ETHYL ACETATE EXTRACT FROM THE MUSHROOM Coriolus versicolor (L.: Fries.) Quelet
The described method of the prediction of retention time can substantially reduce the time required to find optimal conditions in gradient elution.
Efficiency of a solvatic sorption model for the prediction of retention times in linear gradient reversed-phase liquid chromatography working with different stationary phases/Solvaatsorptsiooni mudeli efektiivsus retentsiooniaegade ennustamisel lineaarse gradiendiga poordfaas-vedelikkromatograafias erinevate statsionaarsete faaside korral
6 x 250 mm, 5 [micro]m; Agilent) with gradient elution (at the rate of 1.
Pharmacokinetic synergy from the taxane extract of Taxus chinensis improves the bioavailability of paclitaxel
Contract notice: Supply liquid chromatograph with gradient elution, equipped with refractive index detector, uv detector, automatic injection of sample, communication with different parts
Supply of liquid chromatograph
The combination of low gradient delay volume and high precision gradient formation was useful for high-throughput gradient elution.
pH gradient speeds separation of antibodies: pH gradient chromatography lends itself to the use of shorter, faster columns, enabling a speedier analysis cycle and continuous operation
8 mL/min according to a linear gradient elution program: initial 50% B; 0.
High-throughput assay of 9 lysosomal enzymes for newborn screening
1 x 150 mm) was connected to the XTerra column and the following gradient elution profile was used to elute any organic molecules that were adsorbed on the XTerra column: 100 to 0% water for 30 mins, hold at 0% water for 10 mins.
Ultrapure water provides enhanced protein separation: it is important to use high-quality reagents, especially water, in every step of an experiment when using 2D gel electrophoresis for the separation of proteins
In other attempts SK was fractionated by ammonium sulfate and further purified by gradient elution from a DEAE-cellulose chromatography column (17).
Development of an immunoaffinity method for purification of streptokinase
Table 1: Gradient elution method performed with binary solvent system using as mobile phase.
Antioxidant activity of methanolic and hydroalcohlic extracts of garlic plant
Solvents were delivered according the following program: linear gradient elution from 10% A and 90% B to 85% A and 15% B within 18 min; 85% A and 15% B for 5 min; linear gradient elution from 85% A and 15% B to 10% A and 90% B within 7 min.
PRODUCTION OF TRANS-RESVERATROL IN CALLUS TISSUE OF OKUZGOZU (VITIS VINIFERA L.) IN RESPONSE TO ULTRAVIOLET-C IRRADIATION
A gradient elution was carried out at a flow rate of 1.
Comparative Study of intramuscular phospholipid molecular species in traditional Chinese duck meat products

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