gradient elution

gra·di·ent e·lu·tion

elution in column chromatography in which a changing pH or ionic strength is used to separate substances.
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References in periodicals archive ?
A gradient elution program was applied for chromatographic analysis (Table 1).
Identification, Quantification, and Antioxidant Activity of Hydroalcoholic Extract of Artemisia campestris from Algeria/Cezayir'de Yetisen Artemisia campestris'in Sulu Alkollu Ekstresinin Tanimlanmasi, Kantitasyonu ve Antioksidan Aktivitesi
In the findings of a published study by Geng in the Journal of Science China Chemistry on the two variable-theory of peptides under gradient elution in 2017, a dynamic separation method for the rapid identification and improvement of the selectivity of peptides achieved by simply changing the mobile phase flow-rate (MPF-R).
A New Dimension Approach to Peptide Separation by Prof. Xindu Geng from Oasis Publishers
Initially, a gradient run from 2% to 92% was achieved to determine whether isocratic or gradient elution was appropriate [9].
Simultaneous Determination of Phenolic Compounds in Leptadenia pyrotechnica (Forssk.) Decne. by Using High-Performance Liquid Chromatography (HPLC-DAD-UV)
speciosum extract from three different batches was performed using a rapid high-performance liquid chromatography method under the conditions using gradient elution with a mobile phase of acetonitrile-water, as previous described by our group [8].
Grammatophyllum speciosum Ethanolic Extract Promotes Wound Healing in Human Primary Fibroblast Cells
The water (containing 0.1% formic acid and solvent A) and acetonitrile (solvent B) was used as mobile phase with gradient elution. The gradient was as follows: 0 min 5% B, 0.5 min 5% B, 2.0 min 80% B, 2.1 min 5% B, and 3.0 min 5% B, and then stopped.
LC-MS/MS Method for Simultaneous Quantification of Dexmedetomidine, Dezocine, and Midazolam in Rat Plasma and Its Application to Their Pharmacokinetic Study
The four synthetic food dyes are efficiently separated using an optimized gradient elution in a single run within 15.1 min.
Simultaneous Determination of Sunset Yellow FCF, Allura Red AC, Quinoline Yellow WS, and Tartrazine in Food Samples by RP-HPLC
Gradient elution was performed with (A) 80% methanol and (B) 20% water (pH 3.5 modified by o-phosphoric acid) at flow rate of 1.0 mL min-1 after filtration through 0.45 um pore size.
Liquid Chromatographic Method for Simultaneous Determination of Citalopram with NSAIDs in Bulk Drug, Pharmaceutical Formulation and Human Serum
The fractionation of the ethyl acetate extract was done through a series of gradient elution normal phase gravity column chromatography (GCC) on a 60 - 230 mesh silica gel stationary phase [11].
TOXICITY, ANTIMICROBIAL, AND ANTIMUTAGENCICITY POTENTIALS OF THE ETHYL ACETATE EXTRACT FROM THE MUSHROOM Coriolus versicolor (L.: Fries.) Quelet
Recommendations are made concerning initial column selection and the choice of isocratic or gradient elution approaches.
Liquid Chromatography: Introduction to Method Development
The separation was performed on ODS-C18 column (100 x 4.6 mm, 3 [micro]m) with gradient elution by the mobile phase A, methanol-0.1% formic acid (10 : 90), and mobile phase B, methanol-acetonitrile-0.1% formic acid (50:30:20); the gradient elution processes is 0min (0% B), 15 min (8% B), 45 min (30% B), 60 min (35% B), and 75 min (100% B); the flow rate was 1.0mL/min; the column temperature was 40[degrees]C; and the detection wave length was set at 280 nm.
Neuroprotective Effect of Fagopyrum dibotrys Extract against Alzheimer's Disease
The described method of the prediction of retention time can substantially reduce the time required to find optimal conditions in gradient elution.
Efficiency of a solvatic sorption model for the prediction of retention times in linear gradient reversed-phase liquid chromatography working with different stationary phases/Solvaatsorptsiooni mudeli efektiivsus retentsiooniaegade ennustamisel lineaarse gradiendiga poordfaas-vedelikkromatograafias erinevate statsionaarsete faaside korral
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