gradient elution

gra·di·ent e·lu·tion

elution in column chromatography in which a changing pH or ionic strength is used to separate substances.
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The fractionation of the ethyl acetate extract was done through a series of gradient elution normal phase gravity column chromatography (GCC) on a 60 - 230 mesh silica gel stationary phase [11].
6 x 250 mm, 5 [micro]m; Agilent) with gradient elution (at the rate of 1.
Contract notice: Supply liquid chromatograph with gradient elution, equipped with refractive index detector, uv detector, automatic injection of sample, communication with different parts
The combination of low gradient delay volume and high precision gradient formation was useful for high-throughput gradient elution.
8 mL/min according to a linear gradient elution program: initial 50% B; 0.
1 x 150 mm) was connected to the XTerra column and the following gradient elution profile was used to elute any organic molecules that were adsorbed on the XTerra column: 100 to 0% water for 30 mins, hold at 0% water for 10 mins.
In other attempts SK was fractionated by ammonium sulfate and further purified by gradient elution from a DEAE-cellulose chromatography column (17).
Table 1: Gradient elution method performed with binary solvent system using as mobile phase.
Solvents were delivered according the following program: linear gradient elution from 10% A and 90% B to 85% A and 15% B within 18 min; 85% A and 15% B for 5 min; linear gradient elution from 85% A and 15% B to 10% A and 90% B within 7 min.