The researchers assessed the effect of treatments by counting gonocytes
within the tissue samples.
The present results indicated that exposure of mice to 3 [micro]T of 50 Hz magnetic field from 0 day of gestation affects testicular development by reducing the population of Sertoli cells, destroying gonocytes
, and rupturing tubules or cords in a way that Sertoli-like cells infiltrated into the interstitial tissue.
AB5535) and GC subpopulations, namely gonocytes
(TFAP2C; Santa Cruz Biotechology; catalog no.
Effect of exposure to vehicle or DBP (500 mg/kg/day) on the induction of multinucleated gonocytes
(MNGs) in rat testes at E21.5 (A-C) and in human fetal testis xenografts (D-F).
In treated male fetuses (n = 11), testis weight was significantly reduced (32%), as were the numbers of Sertoli cells (34% reduction), Leydig cells (37% reduction), and gonocytes
(44% reduction), compared with control fetuses (n = 8).
were huge and round and were holding globular nucleus.
(38,39) Normal Sertoli cell function is important for the maturation of gonocytes
in the early testis, and delayed maturation, as already discussed, predisposes to germ cell neoplasia but at an unknown frequency.
Avarbock et al., "Prepubertal human spermatogonia and mouse gonocytes
share conserved gene expression of germline stem cell regulatory molecules," Proceedings of the National Academy of Sciences of the United States of America, vol.
According to recent knowledge testicular physiology is marked by the transformation of neonatal gonocytes
in the period from 3 to 12 months after birth.
First at 2-3 months there is surge in gonadotrophin and testertosterone (Mini puberty) that triggers the transformation of the fetal stem cell pool (Gonocytes
) in to the adult sperm cell pool (Adult dark spermatogonia).
During normal development, neonatal gonocytes
transform into adult dark spermatogonium at approximately six months of age (Hutson et al., 2013).
Approximately 95% of primary testicular tumors arise from embryonic germ cells, either primordial germ cells (PGCs) or gonocytes
, and, as such, are more appropriately referred to as testicular GCTs.