Objective: To determine the frequency of immunohistochemically expression of Glypican-3
in hepatocellular carcinoma on liver biopsies.
Objective: To determine the diagnostic accuracy of Glypican-3
in differentiating hepatocellular carcinoma from metastatic liver tumours while taking histopathology as the gold standard.
AFTV has been shown to induce cytotoxic T lymphocytes specific to glypican-3
, the protein frequently expressed in HCC .
is a proteoglycan that is attached to the cell surface and plays an important role in cellular growth, differentiation, and migration.
(GPC3) may be the most promising among the specific molecular targets for HCC.
Dabeva, "The oncofetal protein glypican-3
is a novel marker of hepatic progenitor/oval cells," Laboratory Investigation, vol.
On staining of hepatocytes (Figure 7(c)), alpha-fetoprotein (AFP), glypican-3
, and arginase-1 were negative.
Antibodies included synaptophysin (Syn), chromogranin A (CgA), CD56, phosphoenolpyruvate carboxykinase (PCK), cytokeratin 19 (CK19), cytokeratin 7 (CK7), epithelial membrane antigen (EMA), glypican-3
, and hepatocyte.
The hepatoid adenocarcinoma component usually expresses AFP and some may also express HepPar-1 [1, 11], arginase-1 , and glypican-3
Immunohistochemical examinations demonstrated that the carcinoma cell was positive for vimentin, AE1/AE3 (focal), CAM5.2 (focal), CK7 (focal), and CK19 (focal) but negative for EMA, S-100, SMA, desmin, H-caldesmon, DOG-1, HepPar-1, glypican-3
, CD34, CD117, PDGFR-Alpha, CK20, bile stain, iron, and mucicarmine.
Some alternative immunological markers, such as HepPar1, glypican-3
, CD34, and AFP, are helpful in differential diagnosis.,,,,
.--Glypican-3 (GPC3) is an oncofetal protein that can be detected in 70% to 80% of HCCs but not in benign hepatocellular lesions, such as hepatocellular adenoma.