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Glycerol is formed from glycerol-3-phosphate by glycerokinase, which transfers the phosphate moiety to ADP, yielding ATP.
Because of the lack of glycerokinase in adipose tissue, the glycerol released by lipolysis cannot be resynthesized into adipose tissue, and therefore glycerol appearance rates in the blood are also a reliable indicator for lipolysis [18].
The following regents were used for the assay: (a) Reagent 1: Reaction solution 4x25 ml [Good's buffer (pH 7.2): 50 mmol [l.sup.-1], 4-chlorphenol: 4 mmol [l.sup.-1], ATP: 2 mmol [l.sup.-1], [Mg.sup.2+]: 15 mmol [l.sup.-1], glycerokinase: [greater than or equal to] 0.4 KU [l.sup.-1], peroxidase: [greater than or equal to] 2.0 KU [l.sup.-1], lipoproteinlipase: [greater than or equal to] 2.0 KU [l.sup.-1], 4- aminoantipyrine: 0.5 mmol [l.sup.-1], glycerol-3-phosphate-oxidase: [greater than or equal to] 1.5 KU [l.sup.-1]]; (b) Reagent 2: Standard solution 1x3 ml [triglycerides].