Ammonium is assimilated into amino acids in a process that is catalyzed mainly by the GS-GOGAT (glutamine synthetase and glutamate synthase) pathway.
One glutamate synthase gene, HvGLU2 (gb|S58774.1), which encodes ferredoxin-dependent glutamate synthase , was also studied in the shoots and roots.
However, the TFP treatment markedly reduced the activity of glutamate synthase
(GOGAT) at 20C and noticeably inhibited glutamate dehydrogenase (GDH) activity during the entire temperature stress period.
Sample Extraction for Glutamine Synthetase, Glutamate Dehydrogenase, and Glutamate Synthase
. One g of plant tissue was hand homogenized in 10 mL of cold phosphate buffer (0.1 M, pH 7.6) containing 2% polyvinylpyrrolidone, 1% [beta]-mercaptoethanol, and 10 mM dithiothreitol in previously chilled mortar using acid-washed sand as an abrasive material .
The present volume considers such topics as flavoenzymes involved in non-redox reactions, mechanisms of bacterial luciferase and related flavin reductases, choline oxidase and related systems, toward understanding the mechanism of oxygen activation by flavoprotein oxidases, glutamate synthase
, and flavoprotein dehalogenases.
Estimates of [K.sub.m] and [V.sub.max] obtained with the glutamate dehydrogenases (GDHs) of Trichomonas vaginalis , Dirofilaria immitis  and Teladorsagia circumcincta [25, 28], and the glutamate synthase
(GS) of T.
Plasma amino acid analysis revealed absence of citrulline and argininosuccinic acid, low normal ornithine, and high urinary orotic acid levels consistent with proximal urea cycle defects like Ornithine Transcarbamylase deficiency (OTC), N-acetyl Glutamate Synthase
deficiency (NAGS), and Carbomyl Phosphate Synthase 1 deficiency (CPS1).
(16.) van den Heuvel RH, Curti B, Vanoni MA, Mattevi A (2004) Glutamate synthase
: a fascinating pathway from L-glutamine to L-glutamate.
Studies on glutamate synthase
from leaves of higher plants.
With increase of nitrogen rate, significant increase in the activities of nitrate reductase, nitrite reductase, glutamine synthetase, glutamate synthase
and glutamate dehydrogenase was noted, which caused an increase in protein and amino acid contents in all the genotypes.
activity was also considerably enhanced by Pb application.
In higher plants, NH + is mainly assimilated through the concerted action of glutamine synthetase (GS) and glutamate synthase