Los genes de interes evaluados para este estudio fueron: Aminomethyltransferase, Glycine dehydrogenase, Glycine hydroxymethyltransferase, [DELTA]-1-pyrroline-5-carboxylate synthase, Ornithine aminotransferase, Arginase, Alanine transaminase, Alanine-glyoxylate transaminase, Glutamate decarboxylase
, Glutamate decarboxylase
1-2, Spermidine synthase, Aldehyde dehydrogenase, ATP-grasp domain-containing protein, Cysteine sulfinic acid decarboxylase1-2, Taurine transporter, HSP beta 1, Sodium- and chloride-dependent glycine transporter (Meng et al., 2013; Cross et al., 2014; Eierman & Hare, 2014).
The following antibodies were purchased from Abcam (Cambridge, United Kingdom): glutamate dehydrogenase (GDH, rabbit: ab34786), aspartate aminotransferase (AAT, mouse: ab93928), glutamate decarboxylase
65 and 67 (GAD65 and 67, both mouse: ab26113 and ab26116), phosphate-activated glutaminase (PAG, rabbit: ab93434), and glutamine synthetase (GS, rabbit: ab73593).
Honnorat, "Respective implications of glutamate decarboxylase
antibodies in stiff person syndrome and cerebellar ataxia," Orphanet Journal of Rare Diseases, vol.
Glutamate can also be synthesized by glutaminase using glutamine as a precursor  and can be converted to gamma-aminobutyric acid (GABA) by glutamate decarboxylase
This decreases the functioning of glutamate decarboxylase
and converts glutamate to GABA causing a glutamatergic deficient state, leading to psychosis.
There have recently been studies suggesting that the occurrence of VIAAT is a good marker of GABAergic signaling in extra-brain tissue cells containing GABA and its synthesizing enzyme termed GAD (glutamate decarboxylase
), such as the pancreatic islets (Chessler et al., 2002; Gammelsaeter et al., 2004; Suckow et al., 2006).
gadA/B encodes for glutamate decarboxylase
. This enzyme catalyzes the a-decarboxylation of L-glutamic acid to yield [gamma]-aminobutyric acid (GABA) and carbon dioxide .
"Lab analysis of metagenomic DNA sequencing data allowed us to demonstrate that microbial glutamate decarboxylase
encoding gene is very abundant in intestinal microbiota as compared to other body sites," Pokusaeva said.
The main inhibitory mediator in the brain, gamma amino butyric Acid (GABA), is formed from glutamate (excitatory) by the enzyme glutamate decarboxylase
Rapid determination of glutamate decarboxylase
activity from lactic acid bacteria by spectrometric method and its applications.
Two genes encode distinct glutamate decarboxylase
. Neuron 7, 91-100.