Blood glucose levels were measured by
glucose oxidase method (Venkatesh et al.
Measurement of fasting plasma glucose levels: Fasting plasma glucose level was estimated by commercially available glucose kit based on the
glucose oxidase method.
All glucose concentrations were measured at a single central laboratory via a
glucose oxidase method.
Each blood sample was analyzed immediately at the farm for glucose by the modified
glucose oxidase method using glucometer (One Touch Basic[R], patent number D456,083, LifeScan, California USA).
Fasting blood sugar was done using the
glucose oxidase method after at least an 8-hour fast.
Estimation: BGL was estimated by
glucose oxidase method (19) using standard kit of Ranbaxy laboratories limited, New Delhi, India.
One week later, blood samples were collected by tail cutting for fasting blood glucose measurements, fasting blood glucose was measured by
glucose oxidase method using blood sugar meter and blood sugar test paper (Johnson & Johnson, USA).
The blood samples for glucose measurements were drawn into ethylene diamine tetra acetate (EDTA) tubes (Becton Dickinson, Singapore) and the separated plasma analyzed by the
glucose oxidase method on a bedside glucose analyzer (GM9D, Analox instruments, London, UK).
In addition to analysis on the Roche Hitachi analyzer, glucose was measured on a Vitros 950 analyzer using a
glucose oxidase method in a dry-slide format (Ortho Clinical Diagnostics) and a Rapidlab 865 blood gas analyzer using a glucose oxidase electrode (Bayer Diagnostics).
Plasma glucose was measured using a
glucose oxidase method (YSI 203 glucose analyzer, Scientific Division, Yellow Spring Instrument Company, Inc.
Serum glucose concentrations were measured in duplicate by the
glucose oxidase method on a Beckman Glucose Analyzer II.
Blood glucose level (BGL) was estimated by
glucose oxidase method (23), using standard kit of Ranbaxy Laboratories Limited, New Delhi, India.
