The sample was centrifuged and serum as stored at -80AdegC for blood assays of glucose by glucose oxidase method
, serum insulin by ELISA and calculation of HOMA-IR.
Glucose was detected using glucose oxidase method
. Insulin was measured using chemiluminescent immunoassay; a fully automatic.
FPG and 2h-PG were measured by the glucose oxidase method
using an automated chemistry analyzer (Synchron LX-20, Beckman Coulter Inc., California, USA).
Plasma glucose, cholesterol and triglyceride contents were measured using the glucose oxidase method
(Lott and Turner, 1975), the enzymatic (cholesterol oxidase) and colorimetric method (Patsch et al., 1976), and the enzymatic (glycerol phosphate oxidase) and colorimetric (PAP) method (Nagele et al., 1983), respectively, using test kits purchased from Junshi Biotechnology Co., Ltd.
The SCGM sensor, which has a small electrode inserted into subcutaneous tissue, can measure IG by the glucose oxidase method
After a 1 h incubation, the glucose concentration in the medium was determined by the glucose oxidase method
(Sigma GAGO 20 test kit), according to instructions.
Plasma glucose was measured using the glucose oxidase method
. Plasma insulin levels were measured using chemiluminescent immunoassays.
Plasma glucose was measured using a glucose oxidase method
Serum glucose level (mmol/L) was determined using glucose test kit based on the glucose oxidase method
(Dialab Glucose, GOD-PAP, Austria), as described .
Glucose oxidase method
for continuous automated blood glucose determination.
Blood samples were obtained from tail vein and blood glucose estimation was done by glucose oxidase method
using glucose kit as per the method of Sood et al!10)