Isolates suspected of being Candida albicans were confirmed by the germ tube
formation method using foetal bovine serum.
Length of germ tube
of the germinated spores was measured, using ocular micrometer which was calibrated by a micrometer slide (Shama and Massoud, 2001).
7) In case where yeast cells did not show sprouting (germ tubes
), the culture was reported as yeast other than Candida albicans'.
To corroborate the killing action of [alpha]-T, aliquots obtained from viable cells reduction experiments at different times (see Section "Inhibition of germ tube
formation") were stained with 10 [micro]l of aqueous propidium iodide (PI) (Molecular Probes, Eugene, USA) solution (10 [micro]g/ml) and fluorescein diacetate (FDA) (Sigma-Aldrich, St.
The results of the ability of the selected antagonists culture filtrates to inhibit germination and reduce germ tube
length of A.
It was observed germinated conidia, germ tubes
and appressoria adhered to the surface of the inoculated fruits.
It was clear that the vigor and growth of the germ tube
in all of the pathogens tested was markedly reduced even at the lowest concentration tested.
It is believed that chlorhexidine inhibits germ tube
formation in Candida albicans there by suppressing the candidal activity .
Under these conditions, germlings fail to grow beyond the germ tube
state of development, due to rupture of hyphal tips and uncontrolled spillage of cytoplasm.
All consecutive non-duplicate Candida species isolated from March to September 2005 were identified using the germ tube
test and a commercial identification kit (Auxacolor 2, Bio-Rad, France).
In addition to variation in trehalose assimilation rates, 3-4 distinct types were apparent in the germ tube