Standardization of counting micronuclei: Definition of a protocol to measure genotoxic damage
in human exfoliated cells.
When evaluating the possibility of genotoxic damage
in lymphocytes obtained from the venous blood of obese subjects exposed in vitro to the drugs Sibutramine or Femproporex, the following results were obtained: after 24 hours of treatment with the drug Sibutramine at concentrations of 5, 10 and 20 [micro]g / mL, the lymphocytes presented greater damage scores (P <0.05) than that of the negative control treatment.
Among different techniques used for the determination of genotoxic damage
, the Comet assay (single cell gel electrophoresis) can detect DNA damage in single cell (Singh et al., 1988).
Micronuclei in oral squamous cell carcinoma: A marker of genotoxic damage
. Indian J Dent Res 2000;11:101-6.
The correlation of the antioxidant status with genotoxic damage
remains a matter of debate.
The observations suggested that eugenol was potent to protect DNA against genotoxic damage
induced by DMBA.
MMS, an alkylating agent, is known for its ability to interact with DNA in vitro and in vivo producing genotoxic damage
. Figure 4 shows the metabolic activation of MMS and the formation of the alkylated DNA .
vulgaris and its primary synthetic constituent thymol against UVA- and UVB-induced oxidative and genotoxic damage
in the keratinocyte cell line NCTC 2544.
When the patients were analyzed together, we could not observe any significant difference between M0 and M1; conversely increased oxidative genotoxic damage
was detected in the group with higher CD4+ T cell count, which could be explained at least in part by the toxicity of cART.
in Solea senegalensis exposed to sediments from the Sado Estuary (Portugal): effects of metallic and organic contaminants.
The pre-treatment protocol allows the evaluation of capability to prevent the mutagen induced genotoxic damage
, while the post-treatment protocol gives information about the capability of the test compound to repair mutagen-induced damage.
The results of the study have shown that lead contamination in the Panasqueira mine area induced genotoxic damage
both in individuals working in the mine and living in the area.