From the 17-kb genomic insert of the SEF1[alpha] in the phage genomic clone
, a 9.5-kb EcoRI fragment containing the entire coding sequence and the complete promoter region was subcloned in pBluescript SK.
To identify soybean genomic clones
corresponding to Mng247, this clone was used as a probe to screen a soybean genomic library of the line L81-4420.
EST and sequenced genomic clone
markers also can be converted to PCR based markers by designing primers from their sequences.
Sequence analysis of Dare genomic clones
confirmed that there are two different, soluble SACPD genes in this cultivar.
Visitors can also access BLAST for comparing genomic sequences and gene products, and a centralized registry of genomic clones
, end sequences, mapping data, and distributor information.
Secondary structure predictions based on the deduced amino acid sequences of cDNAs and genomic clones
, as well as on electron microscopy of the protein (Foisner and Wiche, 1987), revealed a multi-domain structure composed of a central [approximately]200 nm long, [Alpha]-helical coiled-coil structure flanked by large globular domains.
Sequence analysis of the two genomic clones
revealed that soybean F3'H gene consisted of three exons and two introns (Fig.
Detailed physical mapping using genomic clones
proved to be an extremely fruitful approach for understanding one human gene cluster, the CYP2 cluster on chromosome 19 (Hoffman et al.
In many cases, large genomic clones
of specific crop plants will be imperative for the isolation of genomic regions, either up or down stream from the structural gene, interacting with important trans-acting factors controlling gene expression.
thaliana, four genomic clones
(oasA1, oasA2, oasB, and oasC) that encode OAS-TL have been identified and characterized.
In the absence of complete genome sequences, information from expressed sequence tags (ESTs) and end sequences from the inserts of large insert genomic clones
are important sources of sequence information.
A dense molecular marker linkage map for maize using simple sequence repeats, genomic clones
, isozymes, and expressed sequence tagged sites (ESTs) has been developed (Davis et al., 1999) and is still being improved (University of Missouri Maize Genomics Center, 2001).