A hammerhead ribozyme, based on the Haseloff and Gerlach model (9) was designed to cleave a pGUA sequence at nucleotide 5910 within the HIV
genomic clone NL43 (Accession number M19921) (Figure 1).
About 10 to 20 cDNA clones were isolated from the sugarcane leaf cDNA library for each genomic clone.
0-kb EcoRI fragment from one genomic clone was subcloned into the pBluescript SK plasmid vector.
From the 17-kb genomic insert of the SEF1[alpha] in the phage genomic clone, a 9.
To identify soybean
genomic clones corresponding to Mng247, this clone was used as a probe to screen a soybean genomic library of the line L81-4420.
EST and sequenced genomic clone markers also can be converted to PCR based markers by designing primers from their sequences.
Because our objective was to map EST and sequenced genomic clone markers, only the 24 linkage groups containing the 39 RFLP markers (20 cDNA and 19 genomic clones) and four plant-phenotypic loci are presented (Fig.
Century cDNA clones and genomic clones were used to detect RFLP polymorphisms.
In many cases, large
genomic clones of specific crop plants will be imperative for the isolation of genomic regions, either up or down stream from the structural gene, interacting with important trans-acting factors controlling gene expression.
Detailed physical mapping using
genomic clones proved to be an extremely fruitful approach for understanding one human gene cluster, the CYP2 cluster on chromosome 19 (Hoffman et al.
A dense molecular marker linkage map for maize using simple sequence repeats,
genomic clones, isozymes, and expressed sequence tagged sites (ESTs) has been developed (Davis et al.
1990) who found that 65% (15/23) of the
genomic clones evaluated detected RFLPs using BamHI-digested DNA of 19 European cultivars.
