In order to overcome such limitations, technical breakthroughs such as reporter knock-in and gene knock-out
system, production of homozygous mutant ES cells from a single targeting vector, and production of mutant mice from tetraploid embryos are developed.
Using gene knock-out
technology, the company successfully eliminated several genes suspected of limiting expression of foreign proteins in C1 and is in the process of testing the effect of these knock-outs on the expression levels.
The features of these DNA break repair pathways can be exploited to generate gene knock-outs
or introduce defined modifications at the site of cleavage.
The IRES technology is particularly important for evaluating the success of gene knock-outs
or knock-ins in stem cells and for the successful creation of transgenic rodent disease models.
In particular, IRES is important for evaluating the success of gene knock-outs
or knock-ins in stem cells, which is crucial for the successful creation of transgenic mouse and rat disease models.