gene knock-out

gene knock-out

the use of specific techniques that inactivate (‘knock-out’) the function of a GENE. Studying gene knock-out allows gene function to be revealed, by comparing the PHENOTYPE of the WILD TYPE and the ‘knock-out’ MUTANT.
Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005
References in periodicals archive ?
In order to overcome such limitations, technical breakthroughs such as reporter knock-in and gene knock-out system, production of homozygous mutant ES cells from a single targeting vector, and production of mutant mice from tetraploid embryos are developed.
Using gene knock-out technology, the company successfully eliminated several genes suspected of limiting expression of foreign proteins in C1 and is in the process of testing the effect of these knock-outs on the expression levels.
The features of these DNA break repair pathways can be exploited to generate gene knock-outs or introduce defined modifications at the site of cleavage.
The IRES technology is particularly important for evaluating the success of gene knock-outs or knock-ins in stem cells and for the successful creation of transgenic rodent disease models.
In particular, IRES is important for evaluating the success of gene knock-outs or knock-ins in stem cells, which is crucial for the successful creation of transgenic mouse and rat disease models.