I met Voytas at the startup's greenhouse outside of Minneapolis, where he showed me fluid-mixing robots and a tall gene gun
that fires the DNA into a plant cell.
In addition, comparing with other transient expression systems like protoplast transformation (Sheen 2001), gene gun
mediated transformation (Schweizer et al., 1999) and microinjection (Bilang et al., 1993), agroinfiltration has the advantages that it is simple, cheap and can be exploited for intact plant leaves, hence a relative large leaf area can be transformed (Kapila et al., 1997).
In Scotts' newer grasses, for instance, the foreign genetic material comes only from other plants and is inserted with a gene gun
rather than by the bacterium.
Microseeding gene therapy: The therapeutic DNA is directly introduced into target cells using a gene gun
. It is the simplest method of gene delivery.
En el presente trabajo de investigacion se reportan las condiciones optimas de inoculacion por el metodo de biobalistica de un Begomovirus bipartita en plantas de tomate y tabaco utilizando un pistola genica de baja presion (Helios Gene Gun
But once they determine which new DNA sequences are most effective at increasing the plant's bioluminescence--a process that will likely take several months--they'll transfer the DNA to the seeds they'll be distributing to their backers via something called a "gene gun
" a process that involves no agrobacterium.
Once they've hit on the ones that produce the best glowing properties, they will put those retooled DNA into normal seeds with a gene gun
(which is exactly what it sounds like), grow those plants and ship them to backers.
- Global Transfection (Gene Delivery, DNA Delivery, Protein Delivery, SiRNA Delivery) Technologies (Lipofection, Calcium Phosphate, Electroporation, Nucleofection, Magnetofection, Gene Gun
, Viral) Market (2012 - 2017)
Physical methods such as microinjection (16), gene gun
(17), (18) and electroporation (19), (20) are carrier-free gene delivery techniques that employ the use of a physical force to permeate the cell membrane and facilitate intracellular transfer of naked DNA.
DNA cartridges were prepared according to the manufacturer's instructions (Helios Gene Gun
System, Bio-Rad, Hercules, USA).
These include gene therapies (via such in vivo methods as the gene gun
and ex vivo methods involving autografting patients with cultured sheets of genetically modified keratinocytes), protein therapies using intradermal injection of deficient proteins, autotransplantation of marrow-derived mesenchymal stem cells, and enhanced wound healing with thymosin-[beta]4 (1,3).
In the study, snippets of DNA were delivered into skin instructor cells by a device known as a gene gun
. The DNA directed the instructor cells to produce specific proteins, which act like molecular keys.