Alternatively, Thy1 may attenuate lung myofibroblast proliferation indirectly by downregulating receptors such as Fgf and Angtl, which control fibroblast proliferation [52, 53], as we have shown by
gene chip analysis [15] and validated by real-time PCR (Figure 4).
Two-step quantitative real-time PCR (qRT-PCR) was performed to confirm
gene chip results according to Applied Biosystems' guidelines.
Gene Chip Technology Application in the Field of Sports Science Research
qRTPCR data showed similar expression levels as Affymetrix
gene chip analysis (Figure 3).
qRT-PCR was performed to verify the
Gene Chip array of resveratrol-treated RA-FLS.
This
gene chip covers relevant context-specific valid biomarkers and thus could be successfully applied in clinical practice.
While experts are nothing but pleased by the new crop of
gene chips, which cover nearly every gene in the genome, they say that five to ten years from now researchers may shift away from current profiling methods toward new sequencing technologies that are currently in their infancy.
For example, a
gene chip is commercially available that has identified over 300 genes associated with arthritis.
CapitalBio's current products include the LuxScan-10K(A) Dual Color Confocal Scanner for high-density microarrays, the EcoScan100 CCD fluorescence scanner, the SmartArrayer-48 Microarray Spotter and the SARSarray
gene chip for detection of SARS.
The consortium hopes to create a so-called
gene chip that can be read by portable scanners to identify species within seconds, not the weeks or months that current DNA tests take.
Using the '
gene chip' technology, which allows analysis of the function of thousands of genes at a time, the scientists tested DNA from four affected infants along with their parents, siblings and extended family members.
Using the latest '
gene chip' technology, which allows analysis of the function of thousands of genes at a stroke, the scientists tested DNA from four affected infants along with their parents, siblings and extended family members.