gene amplification


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amplification

 [am″plĭ-fĭ-ka´shun]
the process of making larger, such as the increase of an auditory or visual stimulus, as a means of improving its perception.
DNA amplification artificial increase in the number of copies of a particular DNA fragment into millions of copies through replication of the segment into which it has been cloned, a type of nucleic acid amplification.
gene amplification a process by which the number of copies of a gene is increased in certain cells because extra copies of DNA are made in response to certain signals of cell development or of stress from the environment. In humans this process is seen most often in malignant cells.
nucleic acid amplification amplification of a specific nucleic acid sequence, such as to test for presence of a given virus or bacteria in a sample. Types include DNA amplification, ligase chain reaction, and polymerase chain reaction.

gene amplification

n.
A cellular process characterized by the production of multiple copies of a particular gene or genes to amplify the phenotype that the gene confers on the cell. Drug resistance in cancer cells is linked to amplification of the gene that prevents absorption of the chemotherapeutic agent by the cell.

gene amplification

Genetics A process by which specific DNA sequences are replicated disproportionately greater than their representation in the parent source; GS of cellular oncogenes occurs in malignancy, where the copy number is a crude benchmark of tumor aggressiveness. See PCR.

gene amplification

a process in which many copies are made of some genes at one time, while other genes are not replicated. The replicated genes enable enhanced manufacture of product in a short time. For example, rRNA genes are amplified about 4,000 times in oocytes of the clawed toad Xenopus laevis to assist in protein production during egg development. The POLYMERASE CHAIN REACTION can be used for gene amplification in vitro.
References in periodicals archive ?
Significance of EGFR protein expression and gene amplification in non-small cell lung carcinoma.
When gene amplification status was analysed based on HR status in consideration of 50 years age as a threshold value, a statistically significant correlation for ER status was detected in the cases over 50 years old.
Correlation of HER2 overexpression with gene amplification and its relation to chromosome 17 aneuploidy: a 5-year experience with invasive ductal and lobular carcinomas.
The correlation between dual-color chromogenic in situ hybridization and fluorescence in situ hybridization in assessing HER2 gene amplification in breast cancer.
She was being treated with capecitabine and lapatinib in November 2012 at the time of the first blood sample, which had a PIK3CA mutation and gene amplification of ERBB2 and NOMO2 in cfDNA.
This PCR method was also used for detection of gene amplification of other Erb oncogenes such as ErbB1 in cancer tissues (16).
[29], HER2 overexpression by IHC was found in 23% and gene amplification by FISH in 27% of 200 resected tumors.
Among these programs, comprehensive sampling of invasive fungal infections involves nearly 2,000 samples of yeast and moulds, each requiring the highest quality of identifications, and currently requiring gene amplification and sequencing.
Ni compounds exhibit weak mutagenie activity, cause gene amplification, and disrupt cellular epigenetic homeostasis.
The aim of this study was to determine whether 12 TERC gene amplification is detectable by FISH in acute myeloid leukemia (AML) cells.
Detection of gene amplification in MYCN, C-MYC, MYCL1, ERBB2, EGFR, AKT2, and human papilloma virus in samples from cervical smear normal cytology, intraepithelial cervical neoplasia (CIN I, II, III), and cervical cancer