gel retardation

gel retardation

a method of determining protein-binding sites on DNA molecules, by virtue of the reduced mobility of protein-bound DNA relative to unbound DNA in GEL ELECTROPHORESIS.
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Common techniques for this include hybridization of a specific probe to the PCR product after Southern blotting or by liquid hybridization followed by gel retardation analysis (12,14,17).
One aliquot was analyzed by liquid hybridization followed by gel retardation.
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