gel filtration chromatography


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Related to gel filtration chromatography: Affinity chromatography, Ion exchange chromatography

gel fil·tra·tion

separation of molecular sizes by passage of a mixture through columns of beads of cross-linked dextrans or similar relatively inert material of a well-defined pore size range; the larger the molecule, the less time it spends in the interior of the beads, thus emerging earlier from the column than smaller molecules.

gel filtration chromatography

A type of column chromatography in which chemicals are separated via pores according to their molecular size.
See also: chromatography
References in periodicals archive ?
Quantitative analysis of serum lipoproteins (CM, VLDL, LDL and HDL) by high-performance gel filtration chromatography. Chromatography 1996;17:33-7.
Gel filtration chromatography analysis was also performed with the patient's serum, as shown in Fig.
Gel filtration chromatography of serum from the mother and infant, performed with a 40-cm Sephacryl S-300 column (Pharmacia), showed that the majority of the TSH immunoreactivity in the Elecsys assay eluted in a peak with molecular mass consistent with a TSH-IgG complex (Fig.
Validation of a simple screening method for macroprolactin by comparison with gel filtration chromatography [Abstract].
Rapid on-line determination of cholesterol distribution among plasma lipoproteins after high-performance gel filtration chromatography. J Lipid Res 1991;32: 859-66.
The presence of macroprolactinemia was confirmed by the Delfia assay and further investigated by the following techniques (see Table 1 for results): (a) PEG precipitation (2); (b) [sup.125]I binding (5); (c) ultrafiltration (6); (d) gel filtration chromatography (2); and (e) protein G affinity chromatography (5).
The molecular weight of this complex was determined by gel filtration chromatography, as described below.
Using a conservative cutoff of 50%,I found low recovery after PEG precipitation in 30 samples (15.4%); the presence of macroprolactin was confirmed by gel filtration chromatography in all but one sample.