5] Nonstandard abbreviations: TnI, troponin I; TnT, troponin T; TnC, human cardiac/slow skeletal troponin C; cTnI, human cardiactroponin I; cTnT, human cardiactroponinT; AMI, acute myocardial infarction; TnAAbs, autoantibodies specific to human cardiac troponins; I-T-C, human cardiac troponin ternary complex; aar, amino acid residues; I-C, human cardiac troponin binary complex; mAb, monoclonal antibody; ECG, electrocardiogram; GF, gel filtration
The gel filtration
methods are based on the principle that different molecules can be separated on the basis of differences in their size by passing them through a column containing swollen particles of a gel.
In the gel filtration
chromatography, the maximum activity of Reutericin_LHS was observed in the fractions (32-38).
Purification strategies used for bacterial lipase purification Pseudomonas spp Purification Source strategies Pseudomonas Ion exchange Lee and Rhee (1993) putida 3SK chromatography and gel filtration
Pseudomonas Ammonium sulfate Sharon et al.
Table 3: Comparison of four fractions from gel filtration
of n-Hexane crude leaf extract on the mortality (LT50 in h) of termites
In the present study, gel filtration
chromatography was carried out in order to obtain highly purified M13 phages from the supernatant of the inoculated E.
1999, "Purification of antibodies using ammonium sulfate fractionation or gel filtration
The molecular masses of these fractions were estimated by gel filtration
chromatography (Table 1).
In the case of gel filtration
two peaks were observed (Fig.
Vaccine research evaluates the potential of dendritic cells, T27K fractionated by gel filtration
and anion exchange, and a recombinant Ag2/PRA106 + CSA chimeric fusion protein.
Purification was done by ion exchange chromatography using Q sepharose followed by gel filtration
chromatography using Sephacryl S300.
Known volumes of low-speed supernatants and fractions from gel filtration
were added to equal volumes of 2X sample buffer (Laemmli, 1970) and boiled for 5 min.