To assess whether ELISA can specifically detect naturally occurring sLR11, each fraction of human serum and CSF was analyzed for sLR11, following to separation by gel filtration
chromatography; the results were then compared with the immunologically purified sLR11 protein as a quantitative calibrator.
Del Monte banana lectin (DBL) appeared as a single 30-kDa peak in gel filtration
(data not shown) and a 15-kDa band in SDS-PAGE (Fig.
on a fast protein liquid chromatography (FPLC)-Superdex 75 column, which had been calibrated with molecular mass markers, including Blue Dextran 2000.
of PCL on Sephacryl S-200 gave a symmetrical single peak and the molecular weight was approximately 56 kDa (Fig.
EFFECTS OF POE-POB ON GEL FILTRATION
OF LIPOPROTEINS AND THEIR SIZE DISTRIBUTION
The molecular mass of the purified protein was also estimated to be 27 kDa by gel filtration
(data not shown).
We treated each antibody as described above and transferred the antibody to a buffer containing 50 mmol/L sodium phosphate, pH 6.5, in a prepacked gel filtration
column containing Sephadex G-25.
that included fractionation with ammonium sulfate, anion exchange chromatography on DEAE-Sephacel, gel filtration
on Superose 6B, and affinity chromatography with an antibody to subunit-a3 as ligand.
were purified with gel filtration
to give the homogenic heteroglycans Pc-1, Pc-2, Pc-3 and the galactoglucomannan Pc-4.
We found the following evidence for the IgGIgM-vitamin [B.sub.12] complex: (a) an abnormal distribution of vitamin B1, -binding proteins in the serum from the patient, indicated by the results of 2 types of gel filtration
chromatography; (b) high concentrations of vitamin [B.sub.12] in protein G--and protamine-sepharose eluates from the patient, but not from healthy controls or other patients receiving vitamin [B.sub.12]; (c) pentameric IgM in the protein G eluate from the patient, but not the controls, identified by SDS-PAGE and MALDI-TOF MS; and (d) detection of IgG associated with the protein bound to an anti-[mu]-chain primary antibody in an ELISA.
LH, FSH and TSH analytic behaviour after action of plant extracts was studied by both electrophoresis and gel filtration
[ClickPress, Tue Jul 16 2019] Analytical Gel Permeation Chromatography is also known as Gel filtration