gel filtration

gel fil·tra·tion

separation of molecular sizes by passage of a mixture through columns of beads of cross-linked dextrans or similar relatively inert material of a well-defined pore size range; the larger the molecule, the less time it spends in the interior of the beads, thus emerging earlier from the column than smaller molecules.

gel fil·tra·tion

(jel fil-trāshŭn)

Separation of molecular sizes by passage of a mixture through columns of beads of cross-linked dextrans or similar relatively inert material of a well-defined pore size range.

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References in periodicals archive ?

To assess whether ELISA can specifically detect naturally occurring sLR11, each fraction of human serum and CSF was analyzed for sLR11, following to separation by gel filtration chromatography; the results were then compared with the immunologically purified sLR11 protein as a quantitative calibrator.

Development of an immunoassay for the quantification of soluble LR11, a circulating marker of atherosclerosis

Del Monte banana lectin (DBL) appeared as a single 30-kDa peak in gel filtration (data not shown) and a 15-kDa band in SDS-PAGE (Fig.

Musa acuminata (Del Monte banana) lectin is a fructose-binding lectin with cytokine-inducing activity

Gel filtration on a fast protein liquid chromatography (FPLC)-Superdex 75 column, which had been calibrated with molecular mass markers, including Blue Dextran 2000.

A protein with antiproliferative, antifungal and HIV-1 reverse transcriptase inhibitory activities from caper (Capparis spinosa) seeds

Gel filtration of PCL on Sephacryl S-200 gave a symmetrical single peak and the molecular weight was approximately 56 kDa (Fig.

A novel sialic acid-specific lectin from Phaseolus coccineus seeds with potent antineoplastic and antifungal activities

EFFECTS OF POE-POB ON GEL FILTRATION OF LIPOPROTEINS AND THEIR SIZE DISTRIBUTION

Development of a homogeneous assay to measure remnant lipoprotein cholesterol

The molecular mass of the purified protein was also estimated to be 27 kDa by gel filtration (data not shown).

Nebrodeolysin, a novel hemolytic protein from mushroom Pleurotus nebrodensis with apoptosis-inducing and anti-HIV-1 effects

We treated each antibody as described above and transferred the antibody to a buffer containing 50 mmol/L sodium phosphate, pH 6.5, in a prepacked gel filtration column containing Sephadex G-25.

Lateral flow immunoassay using europium (III) chelate microparticles and time-resolved fluorescence for eosinophils and neutrophils in whole blood

that included fractionation with ammonium sulfate, anion exchange chromatography on DEAE-Sephacel, gel filtration on Superose 6B, and affinity chromatography with an antibody to subunit-a3 as ligand.

Circulating proteasomes are functional and have a subtype pattern distinct from 20S proteasomes in major blood cells

were purified with gel filtration to give the homogenic heteroglycans Pc-1, Pc-2, Pc-3 and the galactoglucomannan Pc-4.

Effects of lichen heteroglycans on proliferation and IL-10 secretion by rat spleen cells and IL-10 and TNF-[alpha] secretion by rat peritoneal macrophages in vitro

We found the following evidence for the IgGIgM-vitamin [B.sub.12] complex: (a) an abnormal distribution of vitamin B1, -binding proteins in the serum from the patient, indicated by the results of 2 types of gel filtration chromatography; (b) high concentrations of vitamin [B.sub.12] in protein G--and protamine-sepharose eluates from the patient, but not from healthy controls or other patients receiving vitamin [B.sub.12]; (c) pentameric IgM in the protein G eluate from the patient, but not the controls, identified by SDS-PAGE and MALDI-TOF MS; and (d) detection of IgG associated with the protein bound to an anti-[mu]-chain primary antibody in an ELISA.

Markedly increased vitamin [B.sub.12] concentrations attributable to IgG-IgM-vitamin [B.sub.12] immune complexes

LH, FSH and TSH analytic behaviour after action of plant extracts was studied by both electrophoresis and gel filtration techniques.

Mechanisms underlying antigonadotropic effects of some traditional plant extracts in pituitary cell culture

[ClickPress, Tue Jul 16 2019] Analytical Gel Permeation Chromatography is also known as Gel filtration chromatography.

Analytical Gel Permeation Chromatography Market Research Report, Size, Market Drivers and Key Player