The gag gene
was selected as it is essential for viral DNA integration into the host genome and viral assembly (Chen et al.
Select characteristics of commercially available HIV nucleic acid detection assays, 2011 (12) HIV HIV Internal Amplification Quantification viral Target Control load testing Region Yes Real-time Copies/ml, log Abbott Conserved PCR 10 copies ml, RealTime region IU/ml, or log 10 HIV-1 of pol IU/ml (m2000rt) gene COBAS Conserved Yes Real-time Copies/ml TaqMan 48 region of PCR HIV-1 gag gene
(Roche) NucliSens Conserved No Real-time Copies/ml EasyQ region of NASBA HIV-1 v1.
Novartis AG (Basel, Switzerland) has patented BIV constructs encompassing BIV combination vectors, BIV vectors and BIV packaging vectors and particularly the invention pertains to a three vector system comprising: a) a BIV vector construct including a DNA segment from a BIV genome, a packaging sequence to package RNA into virions; a promoter operably linked to the DNA segment; and a transgene operably linked to a second promoter; b) a BIV packaging vector construct comprising a BIV DNA sequence fragment comprising at least a gag gene
or pol gene of BIV; a promoter operably linked to the BIV DNA fragment; and a polyadenylation sequence located downstream of the BIV DNA fragment; and c) an expression vector construct comprising a gene encoding a viral surface protein.
Comparative immunogenicity in rhesus monkeys of DNA plasmid, recombinant vaccinia virus, and replication-defective adenovirus vectors expressing a human immunodeficiency virus type 1 gag gene
This is not the case for other methods that are based on amplification of the gag gene
and that often have been optimized for the amplification of HIV-1 subtype B.
All vaccines encoded the same codon-optimized SIV p55 gag gene
Phylogenetic analysis of the 13 sequences was performed with reference sequences from HIV subtypes A-D, F, and G from the GenBank [+] database for both the env and gag gene
Feng Zhang, 18, a senior at Theodore Roosevelt High School in Des Moines, IA, was awarded the third place $50,000 scholarship for his biochemistry project in molecular virology titled, "Genetic Functional Analysis of the Moloney Murine Leukemia Virus GAG Gene
Reveals an Inhibitory Element that can be Masked to Control Retroviral Assembly.
The PCR reactions were performed using two set of primers corresponding to positions 917 and 1628 (primers FIV PCR S 2 and FIV PCR A 2) and 1036-1364 (primers FIV NESTED S and FIV NESTED A) of the P17-P24 region of the FIV gag gene
(HOHDATSU et al.
guereza are highly divergent from all known SIV isolates (5), we designed a series of overlapping peptides (16-mers overlapping by 10) across the gag gene
, on the basis of the only available Colobus sequence (5).
HIV-specific peptides, as opposed to full-length recombinant proteins, could also potentially be used to resolve antibody cross-reactivity to the products of the GAG gene
, which is encountered occasionally in uninfected individuals.
Sequence analysis of the complete p 17 region of the gag gene
from the PBMC proviral DNA from each partner indicated only a 1.