subtilis B10 treatment induced high gene expression levels of glutathione S-transferases (GST) and g-glutamylcysteine synthetase (g-GCS) in RAW 264.7 cells.
While, g-glutamylcysteine synthetase (g-GCS) is a rate-limiting enzyme in de novo synthesis of GSH and it plays a pivotal role in GSH homeostasis (Hibi et al., 2004).
2003), and its levels reflect the degree of oxidative challenge placed upon the tissue, g-glutamylcysteine
synthase is responsible for catalyzing the synthesis of g-glutamylcysteine
from glutamic acid and cysteine, and glutathione synthetase then catalyzes the synthesis of GSH from g-glutamylcysteine
and glycine; the second reaction is ATP-dependent (Huang et al.